TCPOBOP.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	TCPOBOP.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49236514
Sequences flagged as poor quality	0
Sequence length	50
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	973989	1.9781843206852538	Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	72622	0.14749622607319438	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGCGTCG	179730	43.388866	10
CGTCGTG	180290	43.371895	12
GCGTCGT	180155	43.335884	11
GATCGGA	181620	43.300896	1
GTCGCCG	116080	43.27345	44
ATCGGAA	180980	43.20191	2
GAGCGTC	180635	43.1784	9
TGGTCGC	113580	43.157417	42
TCGTGTA	180105	43.118607	14
GATCTCG	132985	43.11785	34
GTCGTGT	181730	43.093777	13
CGTGTAG	181000	43.084686	15
TCGGAAG	182775	42.972122	3
GTGGTCG	119765	42.890053	41
AGAGCGT	182535	42.884346	8
ATCTCGG	135405	42.84123	35
TCTCGGT	131920	42.785957	36
AAGAGCG	182205	42.753384	7
GGTCGCC	117390	42.734066	43
TCGGTGG	139320	42.711708	38