G124-G125-M20M38-NEBNext2_CGATGT-_AC8KUAANXX_L008

Basic Statistics

Measure	Value
Filename	G124-G125-M20M38-NEBNext2_CGATGT-_AC8KUAANXX_L008_001.R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16489896
Sequences flagged as poor quality	0
Sequence length	50
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	918486	5.569992679153343	TruSeq Adapter, Index 2 (100% over 50bp)
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA	169533	1.0281022997355471	No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC	61905	0.37541170665964174	No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA	53678	0.3255205490683507	No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG	34977	0.21211170767844748	No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG	33540	0.20339728037096172	No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC	26229	0.15906103956022524	No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC	23119	0.14020100551270911	No Hit
GGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCC	22458	0.13619249023765825	No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA	21805	0.1322324895196428	No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT	21663	0.13137135613226428	No Hit
GCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGG	20843	0.12639861403613462	No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT	19896	0.12065570334706781	No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA	19836	0.12029184416930223	No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA	18614	0.11288124558214314	No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA	18550	0.11249312912585986	No Hit
CCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCC	18317	0.11108014265220352	No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT	18194	0.11033423133778406	No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG	18013	0.10923658948485789	No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC	17357	0.10525839580795417	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	112195	43.802345	1
ATCGGAA	112365	43.633835	2
TCGGAAG	113115	43.34841	3
ACGTCTG	112860	43.20958	15
CGTCTGA	113100	43.152924	16
CGGAAGA	113490	43.11243	4
GCACACG	114595	42.667667	11
TATCTCG	102175	42.612442	39
GTCACCG	110920	42.611824	29
ACACGTC	114825	42.609844	13
TCACCGA	109830	42.52795	30
CGATGTA	108235	42.509373	34
CACACGT	115335	42.462917	12
CACCGAT	109355	42.4564	31
CACGTCT	115365	42.395386	14
ATCTCGT	103110	42.37748	40
ACCGATG	109335	42.35793	32
CTCGTAT	109595	42.35419	42
CCGATGT	109420	42.12928	33
TCGTATG	111740	42.07218	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content