Basic Statistics
Measure | Value |
---|---|
Filename | G124-G125-M20M38-NEBNext3_TTAGGC-_AC8KUAANXX_L008_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 16210328 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC | 539915 | 3.33068522734395 | TruSeq Adapter, Index 3 (100% over 50bp) |
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA | 166896 | 1.0295658422210827 | No Hit |
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC | 55022 | 0.33942558102464054 | No Hit |
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA | 48414 | 0.29866144596210514 | No Hit |
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG | 38651 | 0.23843441045733313 | No Hit |
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG | 31956 | 0.1971335805173097 | No Hit |
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC | 23343 | 0.14400078764599952 | No Hit |
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT | 22026 | 0.1358763376040263 | No Hit |
GGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCC | 21544 | 0.13290292460461012 | No Hit |
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC | 19365 | 0.11946087704085939 | No Hit |
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT | 18562 | 0.11450724501071169 | No Hit |
GTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCG | 17981 | 0.11092311025415402 | No Hit |
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA | 17460 | 0.10770910989586394 | No Hit |
CCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCC | 17040 | 0.1051181691079909 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 64100 | 0.0 | 43.69384 | 1 |
ATCGGAA | 64460 | 0.0 | 43.35868 | 2 |
CGTCTGA | 64925 | 0.0 | 43.119007 | 16 |
ACGTCTG | 64785 | 0.0 | 43.07624 | 15 |
TCGGAAG | 65280 | 0.0 | 42.87144 | 3 |
CTCGTAT | 63055 | 0.0 | 42.834858 | 42 |
ATCTCGT | 59535 | 0.0 | 42.81628 | 40 |
CGGAAGA | 65345 | 0.0 | 42.738194 | 4 |
TCGTATG | 64605 | 0.0 | 42.44323 | 43 |
TCTCGTA | 60490 | 0.0 | 42.21627 | 41 |
CATCTCG | 60210 | 0.0 | 42.21208 | 39 |
GCACACG | 66275 | 0.0 | 42.2012 | 11 |
ACTTAGG | 64340 | 0.0 | 42.145252 | 32 |
ACACGTC | 66385 | 0.0 | 42.077175 | 13 |
CACACGT | 66670 | 0.0 | 41.95862 | 12 |
CACGTCT | 66750 | 0.0 | 41.81792 | 14 |
CACTTAG | 64880 | 0.0 | 41.770744 | 31 |
TCACTTA | 65220 | 0.0 | 41.59307 | 30 |
GAACTCC | 67985 | 0.0 | 41.10837 | 21 |
CGTATGC | 66805 | 0.0 | 41.068546 | 44 |