FastQCFastQC Report
Wed 16 Mar 2016
G124-G125-M20M38-NEBNext3_TTAGGC-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext3_TTAGGC-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16210328
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC5399153.33068522734395TruSeq Adapter, Index 3 (100% over 50bp)
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA1668961.0295658422210827No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC550220.33942558102464054No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA484140.29866144596210514No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG386510.23843441045733313No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG319560.1971335805173097No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC233430.14400078764599952No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT220260.1358763376040263No Hit
GGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCC215440.13290292460461012No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC193650.11946087704085939No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT185620.11450724501071169No Hit
GTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCG179810.11092311025415402No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA174600.10770910989586394No Hit
CCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCC170400.1051181691079909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA641000.043.693841
ATCGGAA644600.043.358682
CGTCTGA649250.043.11900716
ACGTCTG647850.043.0762415
TCGGAAG652800.042.871443
CTCGTAT630550.042.83485842
ATCTCGT595350.042.8162840
CGGAAGA653450.042.7381944
TCGTATG646050.042.4432343
TCTCGTA604900.042.2162741
CATCTCG602100.042.2120839
GCACACG662750.042.201211
ACTTAGG643400.042.14525232
ACACGTC663850.042.07717513
CACACGT666700.041.9586212
CACGTCT667500.041.8179214
CACTTAG648800.041.77074431
TCACTTA652200.041.5930730
GAACTCC679850.041.1083721
CGTATGC668050.041.06854644