FastQCFastQC Report
Wed 16 Mar 2016
G124-G125-M20M38-NEBNext4_TGACCA-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext4_TGACCA-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16529069
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC5487213.319733252973897TruSeq Adapter, Index 4 (100% over 50bp)
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA1653521.0003709222824346No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC544640.32950434171458776No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA491250.29720367190674807No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG371230.22459220177494568No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG275350.16658530495577215No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC241660.146203031761801No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT205980.12461681901140349No Hit
GGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCC193600.11712698398197745No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT181880.11003644548885361No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC173110.10473064151405019No Hit
GTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCG169900.10278860836021678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA670900.043.726041
ATCGGAA675000.043.38652
CGTCTGA678700.043.02160316
ACGTCTG678200.043.01761215
TCGGAAG682100.042.9252133
CGGAAGA685950.042.6426124
AATCTCG610150.042.39115539
CTCGTAT648250.042.25250642
ATCTCGT614750.042.22779540
GCACACG693950.042.13080211
ACACGTC696700.041.989113
TCGTATG676150.041.57229243
CACGTCT703650.041.5712414
CACACGT704300.041.54501312
TCTCGTA629300.041.4536641
ACTGACC671200.041.40299632
ACCAATC621050.041.30717536
GACCAAT634500.041.2773435
GAACTCC706250.041.02058421
CCAATCT629950.040.7445337