G124-G125-M20M38-NEBNext4_TGACCA-_AC8KUAANXX_L008

Basic Statistics

Measure	Value
Filename	G124-G125-M20M38-NEBNext4_TGACCA-_AC8KUAANXX_L008_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16529069
Sequences flagged as poor quality	0
Sequence length	50
%GC	50

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	339130	2.051718702366116	Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC	104581	0.6327095615609082	No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC	47281	0.28604756868036546	No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC	43925	0.2657439448041508	No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC	42928	0.25971214712697976	No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC	24061	0.14556778727222933	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	21255	0.12859163453186626	No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC	19784	0.11969216173034307	No Hit
GCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCC	19003	0.11496715271743375	No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG	17342	0.10491818988716181	No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC	16749	0.10133057100796179	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTGTA	63020	43.3415	14
AGCGTCG	63330	43.337208	10
CGTCGTG	63675	43.267685	12
GATCGGA	64175	43.23979	1
GAGCGTC	63555	43.190056	9
CGTGTAG	63580	43.163372	15
AAGAGCG	63785	43.081272	7
AGAGCGT	64025	43.064884	8
GATCTCG	46685	42.79053	34
ATCGGAA	64560	42.77203	2
ATCTCGG	47420	42.554375	35
GTAGGGA	64335	42.537846	18
GTCGTGT	64790	42.537777	13
CGGAAGA	64500	42.46545	4
TGTAGGG	65125	42.416634	17
TCGGAAG	65370	42.398052	3
GTAGATC	49110	42.27049	31
GTCGCCG	41500	42.129894	44
TGGTCGC	40625	42.00422	42
TAGGGAA	65060	41.95833	19