G124-G125-M20M38-NEBNext5_ACAGTG-_AC8KUAANXX_L008

Basic Statistics

Measure	Value
Filename	G124-G125-M20M38-NEBNext5_ACAGTG-_AC8KUAANXX_L008_001.R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17705690
Sequences flagged as poor quality	0
Sequence length	50
%GC	50

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC	504145	2.8473614979139477	TruSeq Adapter, Index 5 (100% over 50bp)
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA	184005	1.0392421871161193	No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC	58422	0.32996172416889713	No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA	53466	0.30197072240618694	No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG	34927	0.19726426928292543	No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC	28614	0.16160906465661604	No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG	27695	0.15641864282047183	No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT	23648	0.13356158387501418	No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT	22687	0.128133950159525	No Hit
GGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCC	21300	0.12030031023925077	No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA	21184	0.11964515362010743	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	62380	43.684013	1
ATCGGAA	62715	43.393543	2
ACGTCTG	63130	42.80517	15
CGTCTGA	63320	42.791466	16
TCGGAAG	63980	42.621506	3
CTCGTAT	60380	42.358192	42
ATCTCGT	56625	42.267662	40
CGGAAGA	64450	42.23257	4
GATCTCG	56625	42.166676	39
GCACACG	64670	41.908882	11
TCGTATG	63265	41.649902	43
TCTCGTA	57995	41.44661	41
AGTGATC	57125	41.424133	36
ACACGTC	65715	41.25872	13
CACACGT	66245	40.95501	12
CACGTCT	66430	40.79476	14
TCTGAAC	66705	40.520958	18
GTCACAC	64970	40.457394	29
GAACTCC	66865	40.336864	21
GTGATCT	58845	40.336678	37