FastQCFastQC Report
Thu 18 May 2023
G202M11_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG202M11_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14125962
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGATAGGAGTGCCCAGCCAGCCACAAAGGCAGAAGAAAAAACACACG271770.1923904368424607No Hit
AACTATAGCTTTAAAGCCACAGAAAAATATAAAACCAAATAGTGTAGATA265350.18784561362971244No Hit
CTGGAATCAGACGCCCACAGGAGCCAGGCAGGACACACAGGGCCTGTGCT258750.18317336546707402No Hit
GGGCAATGTCACAGGAGAGGCAGTCTGTGTATTTGAGGAGTGTCAGGAAA235190.16649485535923148No Hit
GCACAATCTCTGCCTCAGTGACATGGCCAGAGGGGCTGGTGACATGGGCA226600.16041385358391874No Hit
CTCCTGAGCAAACCCTGCAGGACAGGAGACATGACAAATGGTGGGATTGT195230.13820651648362073No Hit
TTGCAACTCCAACCCCAGAGGATCTGACACCTCTTATGGTGTCATTCAGA193130.1367198920682358No Hit
AGAAGGAGGATGAGGAGGAGGAGGAAGAAGAGGAGGAAGAAGAAGAAGAA182520.1292088991886004No Hit
AAACAGACATCTGACACACAAGAAGGAAAAGGCCACGGAGACCAAGCATT182110.1289186534694062No Hit
GAGCAGAATCAGAGACAGCGACTGCCACAAGAAGAGATAAAGTGGAGCTT158580.112261380853212No Hit
CATTCAACTCATGAACATGAGATCTATGTGGGACAACACACAAGATAGCA150620.10662636640251474No Hit
GGGCAGAGGGCAGAGGGTAAGGGCAGAGGGTAAAGGGTAGCAGGAGGCAG146230.10351861345797193No Hit
TGAGAGACATCTATCCTGGGGAAACACAAATAAACATTTACTAACCCCAG145550.10303723031394252No Hit
CGACCAAAGTACAGGTGATGCAGGGGTAATCAATCTACTGGCTGAATTCC143640.10168511001233049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)