FastQCFastQC Report
Thu 18 May 2023
G202M16_CKDL230013148-1A_H5NKYDSX7_L1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG202M16_CKDL230013148-1A_H5NKYDSX7_L1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120239
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCCTTCACCCTATAACAGACCCATGCATTTCCCTAACAGAAGGCCAG58134.834537878724873No Hit
AAAACCTCCCTACCAGGCACTGTATTCTTTACTCTGTACTGCCAATACAA37453.114630028526518No Hit
CACCTGGGGTTGGCTGGGCAGCCCAGGGGTCCTGACCCCACCTGAGCCAT19491.6209382978900357No Hit
GCCTTACTCTGAGGAATCAAAGCATAAGGTTTTATCACTCTTCATGATCC17941.4920283768161744No Hit
TGGGAGTCTGGGGCCACTGAAGGAAGGCCATGGCCTTGGGGGCCCCTCGC17731.4745631617029415No Hit
CCCAGAGGATCACTGTACGTGTCCCAACAGAAAACCAGGCAGGCAAAGCA17261.4354743469257063No Hit
TCCCATGTCAATCATTAACATAATCCACAGAAATGCACACAGGCCTATCT17231.4329793161952444No Hit
CCTGGAGAGTTCAAGTTGAAACTGTTGTATCTTAGAAATGGATGTTGGGA17211.4313159623749365No Hit
CGGCAATGCCGGAAAAGCTGGCATGACCTGACATTCGTCCATGACCAAGG16971.4113557165312418No Hit
CATAGCCAAAAAAAATATTTTGGAAGAAAACCAGCAGAGAGGTGAGCCAC16521.3739302555743145No Hit
TTAGAGAGGTGATGGAGTTTAAATGAGGCTGCTGGGATGTGTTCTAGTTT15181.2624855496136862No Hit
CTGGGTGGCCAAAGGATTGTGTCTCCTAGGAGAGGAAAATGGAGAAACTG14401.1976147506216785No Hit
TGACGTATGCACACGCCACCGGGGGGGTGGGGCTGGTGCCCGGCACGCGC14141.175991150957676No Hit
TGAAATGTAACATAAATATCTGTGTTTTCTGAAGGTCTTAGACAACCCCT14021.1660110280358287No Hit
GGCCCTCACTATGTGCTCTTTAATCTCACTCTTCCTTTCTTCTCTCCCCT13901.1560309051139812No Hit
ACTGATGAACTGCCTTTCTGTTTGCCAAGGAGACTGCAATAGTTTACATT13331.1086253212352066No Hit
GCACTCATAGGTGGGGTAGATGTTGGGGTGGGTCAACTCAAAGCCCTGGA13261.102803582864129No Hit
TTTCCTCTCTATGTTTGACTCTTGGGAGAGGACTATATGGTCTGCTTACC12931.0753582448290488No Hit
GGCGTTTTGTCGGCCATCTTGAGCTTTGCTCCTCCTTCAGCGGAGGCAGC12701.056229675895508No Hit
ATATTTATAGAAAACTGAAGTGATTTTGCTCATCATTGATAACTTTACAA12181.012982476567503No Hit
TTGTGGTCTTAGACAACCCCTGAAAAAGGGTCATTCGACCCCGAAGCAGC12041.001338999825348No Hit
TAATCCCCATGACTGTTCTTAGAGAACACTTCCCTACACTCTGGCAACAG11550.9605868312278045No Hit
CTCTTCCCTTCACCCTATAACAGACCCATGCATTTCCCTAACAGAAGGCC11150.927319754821647No Hit
TGTTCCCTTGAAGACAACTCCAGGGCCAGGCGAAGCTGCTCCTCAAAGCT10720.8915576476850272No Hit
GGTGGTGGATGTTTAGCCAGAATGCCAGAAGTTTGGGACATTGTCTATGT10410.865775663470255No Hit
CCCCTTGCTCTTCGCAGCTGGGCAAGTGACCAAAGTCAGAGGACCTTGTT10210.8491421252671763No Hit
AACGCTGACACCATTGCATACACTAGCAAGATTTTATCGAAAGGACCCAG10150.8441520638062525No Hit
TCTTCCAGAAGACCAAGGTTCAGTTTCCATGTGGCCACTCACAACAGTCA9690.8058949259391712No Hit
GGTGCCCTTACCATGGGAGAGCCATGGAGGATGGCTGCTGGAGTTCCTGT9690.8058949259391712No Hit
CTTGAGGCCCCCCACCAGGCCTCGAGCAATAGTCTTCACTCTTGGCGTGA9490.7892613877360923No Hit
TCTTCTTGCCCGAGTTCCGCACTCTCTGCAGCCTGGCTGTGATCCCCATC9400.7817762955447067No Hit
CCTTTCTACAGCCACATACTAAACGTAGACAAGTTGGCCTTTTCCTATTG9330.7759545571736292No Hit
TGTTTGAAGGGGACGCTGAAGTTTGTGGCCCAGAACCTTTGTTACATGGT8910.7410241269471636No Hit
GTAGCCTTTTTCCTGTCTGTATGAGAGCTGGACATATCCTATCTATCAAA8620.7169054965526992No Hit
CTAGAGGCTGAAGGGATCCCTATTATTGTCCAACCTCAGACAAAGCATAA8590.7144104658222373No Hit
GAAGGCTGAGTGTGCCTCCATAAGCCTCTCATTCCAAAACATGGAGGCAA8540.7102520812714677No Hit
CGTTTTTTAGTGATTTCGTCATTTTTCAAGTCGTCACGTGGATGTGTCTC8450.7027669890800822No Hit
ATCCAGGACTTGCTGTGGTGGGTGAATTGTATTATGATGATACCAAGTAA8420.7002719583496204No Hit
ATCAATTCATTTTTATTTTATATGTACAAGTGTTTAGTGCATGTATATAT8400.6986086045293124No Hit
TGAGTCTAATTAGAAATTAAAATACACTACTCAGAAATTGGTGCCATTAG8290.6894601585176191No Hit
GCCCATCCTTGGACCTCTCCTTAACAACACTTCATTTCTCTTTAATGGAG8210.6828067432363875No Hit
ACAGAAACAGCATGGAAAGAGGACTTGGGAGAGGGAGGGTGTCCTATGGC8060.6703315895840783No Hit
GCCTTACTCTGAGGAATCAAAGCATAAGGCTGAAATGTTTTATACAATTA7950.661183143572385No Hit
TATCAGATTTTTTTTAACATCTGTTCGCTTTTATGAAATATATCAAGTAC7910.6578564359317692No Hit
GGCTCTCTTGTCTACAGCACAAAAATACAGTGTATCTGTAAATAACTTTC7720.6420545746388443No Hit
GTAGTTGTCGCCTCTGCGGCGGTTGCTTACTCCCGGAACCGGAAACCAAA7670.6378961900880745No Hit
AGAGTAGTTGCAGATCAGTTGCAGAAACAGTACCAAGTCTGCTACGGTGT7660.6370645131779207No Hit
GCCTCCATAACGAACTGGACAATTTTATTACAAGATGCAAAGGATGCCAG7470.6212626518849957No Hit
AGTGATTTCGTCATTTTTCAAGTCGTCAAGTGGTTGTTTCTCATTTTCCA7450.6195992980646878No Hit
GCCATCACACAGCCACCACTGCAGAGCCAGCTCAATGGGGAGAGGGTGGA7450.6195992980646878No Hit
GTACAAAGTCTGGAAATGAATTGGTGGGCAGGGATAATGAGGCGAAAAAG7340.6104508520529944No Hit
TAATGGTGCGTGATGGGATGAGTAGTCGGCTTTCCTGGAGCCTACCGCGA7010.5830055140179143No Hit
TATGTGCCATGGCTGGACTGCCTAGTGTTCACTAGTTTCTACTCTCCAGA7010.5830055140179143No Hit
TTCCCTCTTCCTCATTTTGCTTGTTTCACGCTCTCTACACAGCCCTCCGT6910.5746887449163749No Hit
GGCTCTCTTGTCTACAGCACAAGAATACAGTGTATCTGTAAATAACTTTC6860.5705303603656051No Hit
GCTTTAGGGAATGTGAAAACTTTCCAGTGACTGGCAGCTGCTCTTGGGCC6660.5538968221625263No Hit
GTCACATCAGCTCCTGTATCCACCAAATTTTCTATTTCAACACCGTTTAC6580.5472434068812948No Hit
CGCACCTGTCAAAATGTGCACTGGCTTCTGCAATCTCCTCTCAGGATGGA6440.5355999301391395No Hit
GGCCTCTTCTTCCTGGTTTCTTTCAGTTTCTACTCTCCAGAGCAAACACC6340.5272831610376001No Hit
TTTTCCTGCTCTTCTGTAGGGTTTCAGCATGATGTCTGATGGAATTGTCT6280.5222930995766765No Hit
AAATGGCTGCACCGCCGGGTCCGAGGGTCACTCTCCAGGCGCCCTTGACA6170.5131446535649831No Hit
CACGGAGCTGCTGACTACTCCAACCTCGGCGCTGCAGCTTAGGCCTTCAG6070.5048278844634437No Hit
CCCCTGACCCCTATCTCTCCCGGAATTAAATTATGTCTTTTGTGGGAAAC6030.5015011768228278No Hit
TGTGGACAATGTAGTTCTAATCCACTGTGTGGTCTTAGACAAGACTCTTA5880.48902602317051874No Hit
GGGACGCAGGGGGGCGGGCCCTGGCCCGCACATGCGCAGACATACTGCTG5800.48237260788928715No Hit
CTTGCGCCATCAGGCCCGGTGGGCGGGACATGTCTAAGCGGGGCGGGACC5780.4807092540689793No Hit
TCCAGTCTACAACAGCTCCTAATGGAAGAAGCACAGGACATTACTTAAGT5730.4765508695182096No Hit
CCAGCCACATGGAATGGTATAATCAGGTTATATGAGTTACAAGCTAGATG5620.4674024235065162No Hit
CATGGCTTCCCAGGGGTTCTCCCTGCTTCCCAAGTCTTCAGCTTCACCTG5590.46490739277605436No Hit
TGACCCCTCTCTCAGTTTCTCCATCTGGGAAATGGGAATAACAAGCCTTC5440.4524322391237452No Hit
GGTGGGCTGAGGGGTAGTGACTCAGCAAAGGAAGATGGTTTGAAATTCAG5370.4466105007526676No Hit
AGAACACAAGTACTGTGCTTCTATGGCAGTGGACCTGATGACCAATTGCT5280.4391254085612821No Hit
ATCCAAAGTTTGCTCTTAGAAAGTATTTCCCTTTGTCTACTCATTCTCAG5170.4299769625495887No Hit
GGGGACAAAGGGTGACATGAGCATGGAGAAAGAGGTCAGGTGACTACATC5140.4274819318191269No Hit
CATCCCCAAGCTGGGCCCGCAGTTCCCCTCGCCGCAGCAGCGCACTACGC5070.42166019344804934No Hit
TGCCCTTCCCCTCAGCTCATCACAGCTCAGCTGAGGCCCATACCCCTCCC4910.40835336288558616No Hit
TGTGCCTTCTCCTCGCAAAACATGCTCGCACTGGAATCCTTGGACAGCTC4900.40752168597543226No Hit
CTGGAAGTCATAGCTGGAATGTCTGTGTCATCTGCAGAGCCAGGAAACTG4870.40502665524497045No Hit
TCCTAAGGCTCCGAGACAGTTCCATATCCCAGTTCTGTGGGAATGCTTAT4830.4016999476043547No Hit
TGGTGATAGATGATCCAGACTCCGCCAGCAAAATGACCAGCTTTTTAAAA4810.4000365937840468No Hit
ATGAACAAACAAAAAATGCACACTCATACAAAATATTTTTGAGATGGGTG4750.3950465323231231No Hit
GAGAACTTCTGGTGGATTCACCATCCCTGATCTCAAACTGTAATACAGAG4690.39005647086219947No Hit
CCGTGGAACAGCAGCTGGCCCAGCTCAGGCTGCAGGAATGTCCAGGTCCA4650.3867297632215837No Hit
TTCAAACACTAAAACTGCAGCAATACATCATTAAAAGCTGAAAAAATGAA4560.3792446710301982No Hit
AAACCATCCCTGCATCCCTGGGATGACTCCTCCCATATACAGCCACCAAA4560.3792446710301982No Hit
TCAGGAGCTTGTTGGTCTGGGTCCATCTGCTCACCACCCACACATGACCT4520.37591796338958244No Hit
TCCATTTTCAGGTCTTGGGAAGTTAAAGTAAAACACTGCTGAGGAAGCCT4460.37092790192865877No Hit
TGGAAAAATTCTCAAAGTCAAGATTAGCTGAGGATATACCCAAGAGATTA4420.367601194288043No Hit
AGGGGAGTGGTATCAAAGTTATTGAATTCTCAATTCTTCAAAGGCCAGCC4350.36177945591696536No Hit
GGATGTTCTCCTGAGCAGATCTGTAGGCAGCACACACTTAGTAATGATAT4350.36177945591696536No Hit
TCTGGGATCAGGGACTCGAGCCAGCTCGGCTGGGCTGTTGGTTTTTGGTT4160.3459775946240405No Hit
TTTCCTCTCTATGTTTGACTCTTGGGAGAGGACTATATGGTCTACTTACC4110.34181921007327076No Hit
TGTGAGAGCTTGGGCACAGTCATTGCATGAATCAAGGTGGACTCTACAGC4100.3409875331631168No Hit
TATCATCCTGAGTGAAGTAACCCAATCACAAAGGAACTCGCACAATATGT4080.33932417934280895No Hit
GGAGGCATTGAGAAAGTCACTGGAATAGGGCTAGGTAGAGCGGCACGTAC4060.337660825522501No Hit
GCTTACAGTCTATCTGGAAAGACCTCAGTCAGAAGTGTGCTACAGAGATG3880.32269064113973No Hit
TCTGGTTTCCACATTTGGAGATTGCCAGAGTAGGGCTTACAGGTTCTACA3820.3177005796788064No Hit
GTCGTGCAGTCCCACTTGAGCTGTTGTAGACTGGAAGATCGGAAGAGCAC3780.3143738720381906No Hit
TGGACAGTTCCTTCAGCTGTCACAGGAAGAAGTCACGCGTGCACGCAGCC3610.30023536456557354No Hit
TGTTCAAACACTAAAACTGCAGCAATACATCATTAAAAGCTGAAAAAATG3560.2960769800148038No Hit
AGCTACACCCTGGAAAGACCAGCGCAAGTGAGTCCTGGGGTTCAGAGTCT3550.2952453031046499No Hit
CCACACCAACTGCTCTTAGGGGTCAGGGGAGATCGGAAGAGCACACGTCT3540.29441362619449596Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTTTAGGATGTGAAATATGGGGAGGAAAACTGAAAAAGGTAGAAAATT3530.293581949284342No Hit
CCTAGCACAATTCACCCACCCCACCTCTACCTCCAGCTTGTAACCCGTGA3440.2860968570929565No Hit
CACCTCCACACGTTCAGGTCTAGATCCAAGTACACAGCTCTAGTATCAGC3430.28526518018280256No Hit
CTACAGAGTGAGTTCCAGGACAGCCAGGGCTATATAGAGAAACCCTGTCT3400.28277014945234075No Hit
CACGCGGTGGGCAGGGCCCTCCCATGCCAATCATCAATCAAGACAATTCC3210.26696828815941587No Hit
CATCCACTTGACGACTTGAAAAATGACGAAATTACTGAAAAACGTGAAAA3210.26696828815941587No Hit
CAGAAACCCCCTACCACTGGTGTTAGTAGAGTTCCATGGAAAGGGATGGC3160.2628099036086461No Hit
GTATGCCCGTGTGCTTTGTGAATACACATGCTACATAAACAGAGGTGGCA3100.25781984214772247No Hit
GAATTATTCTTACCAATGTGGCAGTAATCTGCTTTTAAAAGAAACACACA3090.2569881652375685No Hit
CACTGCCAGCTCACCCTTTCCATCTGTGTGAGTCACTGAAAATGTCAACC3060.25449313450710664No Hit
CTGCTACTCCTCGGGGCCGCCGCGGCCGCCGCTTCGCAGACGCGGGGCCT3050.25366145759695274No Hit
GGTCAGAGGGACTCTGTGTCCTTGTAGCTGTTCCAGTGAGCCAGCTAATT2910.24201798085479753No Hit
GCCGTCCCCGGGTTGGGGGGCGGGGTGACAGAAACCACGTGGCTCCTTCC2860.2378595963040278No Hit
GGCTCTCTTGTCTACAGCACAAAAATACAGTGTATCTGCCAAGGATATAT2850.23702791939387385No Hit
CCCTCTGTACTCACTCCAAGAGATGAGAGAGATCAATAATCAATTAATCA2820.23453288866341204No Hit
TCCCCTAGAGTCTTCTCATTCAGGAAACACTTGAGCACTTGGAGGAAGAC2810.2337012117532581No Hit
GTTCTCGTGTTCTCTCTGAAAATAATCTTCTGAGAAAGATAGACCGATAA2760.2295428272024884No Hit
CCCATGTCTGTTTCAGGAAAACATCCTGTAATATAACTGACTTTCCTGAA2750.22871115029233446No Hit
TGATGGCTGGAGTGGCGACATCCAGCCATAGTAATATTTTGTGAAAAAGT2720.2262161195618726No Hit
CAACTGTATGTACCTCCATCCGTCAGAAGTCAGCCTCAAGCAACCCCAGT2710.22538444265171867No Hit
CTCACTAGCAGTGAGTCTGGGTCACTCTCTGGGAGCCTGGCTTCAGAGGC2700.22455276574156474No Hit
CCCTGTGGCTTCCTGTCATCTCTCCCCTGGAGCCCAAAAGCTGGGGAAAG2660.22122605810094895No Hit
TACTTTTTGTGGAAGAAGGAATACCGGTGAGACACATCACCATACTCTGC2640.21956270428064106No Hit
GGCTCTCTTGTCTACAGCACAAGAATACAGTGTATCTGCCAAGGATATAT2620.21789935046033315No Hit
ACTGACCCTCTCAGTTTCTCCATCTGGGAAATGGGAATAACAAGCCTTCT2590.21540431972987134No Hit
CCCCCTCCCCCGTTCATTAAAGGACAACTGACTAATCTGGAACTTTATCA2520.20958258135879373No Hit
TGACCTTGGAAATGGGGAAGGGGTCCTGCCGCGGGTCCTTCCGGGGGTGA2510.2087509044486398No Hit
ACTGACCCCTCTCTCAGTTTCTCCATCTGGGAAATGGGAATAACAAGCCT2500.20791922753848588No Hit
CCTGTATCCACCAAATCTTCTATTTCAACACCGTTTACTTGAAATTTTAA2480.20625587371817794No Hit
TGAAGGGGAAGAAACCCCAGCCTCTCCTCCTTGATTGTCTGAGACATGAC2450.20376084298771613No Hit
GCCCCTATGCTCTCTAACACTGAAACCTGAGCAAACAGGTTTGGGAAGTT2410.20043413534710036No Hit
ATGATGTTTTGATGGAGCCTTATGGGAGGAATAGTGGAAGGGTCTTTTGA2290.19045401242525303No Hit
CCAGCCCTGGCTTCCCTGTTCAGATGGGAGGTATAACACAAAGGTTTATT2250.18712730478463727No Hit
TGTGCAATGGGCTGAGAAGAACAAACATGGACTTGCACAATGACACAAGA2130.17714718186278994No Hit
GCTGTGCTCACTTGCAGGAGTCTCTCAGGGTTAGGCCTATGGACTGTTGA2100.1746521511323281No Hit
TGATGGGGATTGCATTGAATCTGTAGATTGCTTTTGAGACTTGAAGTTTT2070.17215712040186626No Hit
TCTCAGTTTCTCCATCTGGGAAATGGGAATAACAAGCCTTCTATTGAAAC2060.17132544349171233No Hit
CAGATACCACTAGGCATGGTGTGTCACACTTGCAAGCCCCACATGAAGAG2050.1704937665815584No Hit
TAACATCAGCCCTAAGATTAACAATTAACAAAGATAATCTGATGAAACAC1980.1646720282104808No Hit
CCAACAGGGCCTGGTCATAAAGCTCAGCAGCCCCGGGCTCCATGGCTCTG1930.16051364365971107No Hit
TGGCCAGAAAACTGAGCAAAGGAAAGGATCTTGGGTGGAATCTACACACA1900.15801861292924924No Hit
TATGTGCCATGGCTGGACTGCCTAGTGTTCACTAGAGTAAGGCAGATCGG1900.15801861292924924No Hit
CCATGCAGAGAAATACTGACCGATTTACTGGCTGATTGATCTCTTGGCAG1890.1571869360190953No Hit
AAGGCTAGCCTGGTCTACAGAGCAAGTTCCAGGACAGTCAGGGCTGCACA1880.15635525910894135No Hit
CCGAATGGCTGAGAAACACCTTAAAATATCATCATCAGTCTTAATCATCA1830.15219687455817166No Hit
TAATGGTGGGCAGGCAATTGGATGGAGAGTATAAAGGCATCTCTCACTAT1810.15053352073786375No Hit
TCATGCAATTTGACCAGATTCAGATGAGCCTCACCATCTGTCTTTCTTTT1800.14970184382770982No Hit
TAGCTTCCATTGTCAATTTGATACAGCCCAGAGTCACTTTAAAAAGGGAA1770.14720681309724798No Hit
ACCTCCCAGAAACCCTCAGGCCTCCGCTAGTTCCCGAACCTCGCGAGACC1760.14637513618709405No Hit
GCCTGGAGGAGCTTCTCTAAGCCACGCCTACCAACTAACCAACTGGCCAA1720.14304842854647826No Hit
AAATCTATGAAGATCAATAAGAAGTAAAAATCTAAAACATGGGTTACCAT1610.13389998253478488No Hit
TCCTTGTAATTCAAGAGAGTTAGAGTCCATAACCATCATGGCAAGGAGCA1550.12890992107386123No Hit
AACACCTATGTAACAAGCCAGGCATGGTGGCACATACCTGCAGTCCCAGA1550.12890992107386123No Hit
ACCCGGGCCGCCTGGGTGAAAACCAGGAATCCTAACCGCTAGACCATATG1450.12059315197232179No Hit
AGCTACACCCTGGAAAGACCATGCTCAGAGGCTTCTAGAAATGATGACAA1420.11809812124185996No Hit
ATGGAGACAGATTGTGTCAGACGTGCACAGCACACAGTAAACGGGATGTG1420.11809812124185996No Hit
GGCTTTCAAGTACAGGACAGCATCAACTAACAGAAGGTCATTAAATGAGG1380.1147714136012442No Hit
CCCTTGGGGTCCTTCTGAAGGGCTATTAAGTTCCCTAGGAAAGCCTGTCA1380.1147714136012442No Hit
TGAAGACGTGGGAAGAGCTCAATTTTTACAGTTGCGGGGCTGGGTCTCTG1380.1147714136012442No Hit
GCCACATGTGTGCTCCCTCTCCAGGCTCTGTGCAGCAACTCTGTCCACAG1360.1131080597809363No Hit
TAATGGAGTCTCTCAGGGTTAGGCCTATGGACTGTTGACCTTCACTCATA1360.1131080597809363No Hit
TTTTTCAGTTTTCCTCGCCATATTTCACGTCCTAAAGTGTGTATTTCTCA1340.1114447059606284No Hit
GGAGATCCTGAAGGCAGGCACAATTGAGAGCTCCCTTCTTGATTAGGACA1290.1072863214098587No Hit
AACTCTTTATGCCTCCGTAACTTTTCCATCAATGGACATAGTGTCCTATG1260.10479129067939687No Hit
AGCAAAAGTAATCGATACTAGCCTCTGGACAGAGAGGAAAGGACAGACAA1250.10395961376924294No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)