FastQCFastQC Report
Thu 18 May 2023
G202M16_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG202M16_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120239
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGCATTGAGAAAGTCACTGCCCTCCCGCACAGCTCTGGCCAGCAGGG89107.410241269471636No Hit
GAAGCAGGGTCTGGTGGAAGCAGGATTCAGTCTGATGGCCCTCTGGCGCA26082.1690133816814847No Hit
ATGTCTGGAGCCGAAAACGGTGCTGCCTCAGAAGCTCTGTGGCTCTCGTC24862.0675487986427035No Hit
GGATTTCTGAGTTCAAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACA19521.6234333286204976No Hit
CCATCTTTAGGGAAGGTATCACATGGGCTTTAAAAAGTGTTTTGGTGTAG19441.6167799133392657No Hit
TCAACTAAGGTAGTACTTTTCCACTAAGGCCAAGAATTTCTAAGTAAGCA19401.6134532056986501No Hit
TCTATAGATATTTGCTCTTAACTTGGGGTAGTATTTTCCCACAGAAACCT17491.4546029158592468No Hit
AAGGAAGCTAGAGCCAAAGGAGTTCAACCAGGAGAACTTTCTTCCTGCTG17291.4379693776561682No Hit
GCTGCCTCTTCCTTAGCCACACTTCAGTTTGGTGGTATGAATAACCATTT17151.426325900914013No Hit
CCCCAGAAACGGCTGGCCTCTGTATTCCACACCGTCACCCGTGCAGCCTG17151.426325900914013No Hit
TCTAATTCTTCCCCGTTCTCATTCCATCTACATTTGCTCCTCCAATTCTA17041.4171774549023195No Hit
TAGTCCTCTGGGTCTAAGGGCAGTTGGGAATGCTGTGTGTTTGCCCAGCT16691.3880687630469315No Hit
GAAGCAGGGTCTGGTGGAAGGAAGTTAGGTCAGGAAGGATGCATCCTTAA15061.2525054266918387No Hit
TTTCCTTCGGACCTGCGTGGACCTCATTATCTCAGAAACCGAGGTCCGGC14921.2408619499496836No Hit
GCTACAGGAGAACAGAGCCCTGTTCAGGACCCTCTTTGACATTGCTAATC14401.1976147506216785No Hit
ACTGACCCCTCTCTCAGTTTCTCCATTTTCCTCTCCTAGGAGACACAATC13701.1393973669109025No Hit
CATGGCCCTGAGACTCAACGCTGGTCCTCACTTCAACAAAATGAGACAGA12801.0645464449970476No Hit
GGAAGTCACGCGGCCCCGGCGCCGCGGAGGTCTCCGTCTCGGCGTTCGGC12751.0603880604462779No Hit
GTGTAGAAGAGCTAGCCCCATGCCTTGCCTGGGCATCCCATTTCAGCTGG12691.0553979989853541No Hit
CTGGGTGGCCAAAGGATTGTGTCTCATATGTAGGAAATTTTTAAAAAGAA12531.042091168422891No Hit
AGAATTTTATGTGATTCTCTAAACCATGGTGATAGTGGTTGCTACAAGGA12531.042091168422891No Hit
CAATATACGGAAATCCATCAACGTAATCCAGTATATAAACAAACTCAATG12191.013814153477657No Hit
ACAGGTACATATGTCTTGCTGGACTTAAATGCACTATCCCTTAAAATAAC11470.9539334159465731No Hit
GGTGGTGGATGTTTAGCCAGAATGCCAGAAGCTTATGACATTATCTGTGT10530.8757557863921024No Hit
ATGGGCTTGGCTCCTCCCCAAGCCTTCTCACTGGTCTGCTCTCCCCTCAA10520.8749241094819485No Hit
ATCCAGGACTTGCTGTGGTGACACAGTAGAAAACTCTACAAGGGACAAAA10410.865775663470255No Hit
TGTGCTGCTACCTACAACAAGCACATTAACATCAGCTTCCACAGGTAAAC10190.8474787714468683No Hit
TAGCTCCTTGGCTACTTTCTCTAGCTCCTCCATTGGGAGCCCTATGATCC10160.8449837407164065No Hit
ATTTTATTTGTGTGAGTGTTTTGCTTATGTGGATGCCTGTGTACCACATG9680.8050632490290172No Hit
CTTGTTGTTCATGGAAATAAGTGTGCAGAAGACAGTCTGTCAGGGTCAAA9610.7992415106579396No Hit
CCAGGGAAGAAGATTCCGGCACCCTGGGCAAACACCACGAGTGTGCAGAA9520.7917564184665542No Hit
CTGGAGAGTCAGATGGTCCATTTGTTTTTCTAAACACGTGTAAAGGAAGT9390.7809446186345529No Hit
TCTGCAAGCATAGCACAGAGCAATGTTCTACTTTAATTACTTTCATTTTC9160.7618160497010121No Hit
TCTCTATCTTGGGTGGGGCAGAGGAGAGAGGCTTGCACTTCTTCTCCAAG8810.7327073578456241No Hit
TCATTTTTCTTTGCAGTTGAGTTGTGCACACGCGGAATCTGGCTAATTTT8640.7185688503730071No Hit
CACTTTAGGACTTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATT8440.7019353121699282No Hit
CACCACCTTCTATTTTATTGCTCATGTTCTGCCCGCCTCCCTCCACCCTC8430.7011036352597743No Hit
GCTCTTAGAGTCTGGTTCCCAGCACCAGTGTCAGCCAGCTCACAACTGCA8410.6994402814394663No Hit
GACTCCCTTCGTGACCCATGTTAAGGCGAGTTAGCCTGAGATGGTCTCTC8330.6927868661582348No Hit
TGTGAGGAAAGTCGCCAGGATCTCCTGCTCAGTGTTTTTACTGCCTATAC8330.6927868661582348No Hit
GGCACTGTGCCAAGGTCAGGAAAGTACAGATTCTGTGCCCGAAATCACCA8110.6744899741348481No Hit
TAAGCAATTGTTTTTCTCTTGGCAAGAAAAGAAAAAAAAAATCTCCATTG8090.6728266203145402No Hit
AGGAGCTGGGACAAGATTGGTCTCATTCTGCACAGCCCCACAGCAGAGGT8090.6728266203145402No Hit
AGGCTGAACTTCTAAAGTCCTTCCAAGTACATTTCTCAACCAAAACAAAC8020.6670048819434625No Hit
TGTTGATAATTTTGCCACATTTGCAATATCCTTGTTGAAATTTTTTTCTT7860.6536980513809995No Hit
GCAGTGCGCAGGCGCGGAGCCTCCCCAATGAGGGCACGCGTGACCAGGCT7780.647044636099768No Hit
TATGTGCCATGGCTGGACTGCCTAGTGTTCAAGGAAGTCACAGGCCTCTT7780.647044636099768No Hit
CTTTAGAACATGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA7510.6245893595256115No Hit
CTGGGGTCCCTTTCCAGTCAATGAATTTTCCAGAAAAATTAAGAGGCAGA7430.6179359442443799No Hit
GTAGCTTTTATATCTACTGTGAAAAATTAAAGCAAACTTCCCTGTTCTCT7340.6104508520529944No Hit
TAAGGTTTTATCACTCTTCATGATCCAGACTATCTTTAGGTAAGAAGAGA7070.587995575478838No Hit
CCAGAAGAGGGAGAGAGAGAGAAGGCGAATGTTCCCCCAGCTGTTTCCTG7040.5855005447483762No Hit
TATTCTTTTCCCGGTTGCTAATGAGATACATTGATTTTAGTATCACCTCC6870.5713620372757591No Hit
CTCGGAGCCCACGCTGATGCAATCGGAACCGGCCTCCCGCTCCTGGGTTA6830.5680353296351434No Hit
AGAGAAAGACTGATTTCATTCACAGTAAAGTTTCTTTATCGAAGCTAGTG6800.5655402989046815No Hit
GGCTACCATCAGACCAAGAGGGATATCCAAGACAATATCTTGTCACCAGG6750.5613819143539118No Hit
TCCCCGACTGCGCGAGCGCGACACCGGGGCCCTTTTACGACAGTTCGGAA6660.5538968221625263No Hit
GCCTTACTTTATTTTCTCTCTAGGCTGAAATGTTTTATACAATTAAAAGC6450.5364316070492935No Hit
TCCTGATGAGTGACCTCCTAGCATCTCACCAGGCCCAAAAGTGGCACTTT6400.5322732224985238No Hit
TGCCCGCACTCTGATTGGGCGCATCTCAGAACCCTGGGCGCTCATTGGCT6180.513976330475137No Hit
CAGTGCCTTGGCGGTCCCAGACTCCAGCTCGGTAAAGGTGCGCGCGGGGA6050.5031645306431357No Hit
AACGGTGCCTCCCGCGCCACGCCCTGCCACGCCGCACCAACCAACAGCTC5960.4956794384517503No Hit
AGGACACAATCTTGAAGAGGTGCACTCCAGTGAATTTTGGAGAGGTGTAT5870.4881943462603648No Hit
TTTGTTTAAGAAGTGAAACCTCATTACACCCGGACCTCTTTAATTAGTTA5570.4632440389557465No Hit
CTATGTCCGCCACAGTTCCCAAGTAATAACATGCATGGAGGCTTACTTAT5490.4565906236745149No Hit
TGTCATAAACAAATAGCAGACAGACCAAACAAAATGGACAGTGCACTGGA5390.44827385457297547No Hit
GACTTGCAATGCCGTGCTATGTATCACATGAAATCCCATGATGCTTTGCT5310.441620439291744No Hit
CCCAATACTGGAAGATTTGATGTAAAACTGGAAGCGCTCAACCTGAGCGC5190.43164031636989664No Hit
TGTTTTGGGATTTTAGCCAGTTGCAATGGAGCAATGCAGGCTGCCAACTG5040.41916516271758747No Hit
GCGGGGACCATCCGTGGCTCTGCTCCAATGGGCCCGGGGTGCAAGCAGAA4970.4133434243465099No Hit
ACAGGCAGAGCCAAAGAGAGCATTGGTGTGGTCGGGACGCTGCCCTCCCT4940.410848393616048No Hit
CTGGAGAGTCAGATGGTCCATTTGTTTTTCTAAACACGTGTAACTAAAGA4900.40752168597543226No Hit
GGCTTTATTGCCCTGGGTCTGTGGTCAGACAGAACACCATTACAGGACAC4860.40419497833481643No Hit
GCCTGTCCTGTTACAACCCACCCACAGCCTCCGTTGAAAGTATCCGAGTC4850.40336330142466253No Hit
GCAATGCTGAGCCAGGTAACTGTCCCAGTACTTAGGCTCTGTCCAAACAC4850.40336330142466253No Hit
AGGCTCCCAGGAGATATTAGCTGAAATACCCAACAAAGTGGAGAGAGAGC4830.4016999476043547No Hit
CCCCTGACCCCTAAGAGCAGTTGGTGTGGAGATCGGAAGAGCGTCGTGTA4700.39088814777235337Illumina Single End PCR Primer 1 (100% over 21bp)
TCTTGGTTGCCATTTTGCCCTATTGACAGTGTCCTTTGCCTTACAGAAGC4660.38756144013173766No Hit
GGTGTTCCCTGTGCCTGTTCCCTGGATGTTGGCTGGAGTCCATGTGACTC4650.3867297632215837No Hit
CAACAGCTCCATGAACCGGGGCATCCTGACCCCACTTTACGACTGCCAAG4610.38340305558096793No Hit
CCACACCAACTGCTCTTAGGTAATGACTAATCTCAGTTTCCCACAAAAGA4550.37841299412004425No Hit
TGAGCTTCTGCCTCTGTATTTGTCAGGCTCTGCCGGAGCCTCTCAGGAGA4510.37508628647942843No Hit
CATATCATTACTAAGTGTGTGCTGCCTACAGATCTGCTCAGGAGAACATC4440.36926454810835085No Hit
GGAGCCATAGAGAAGAGAATTGAACTGTATCAAACTGGGTAGAAAGAGAA4420.367601194288043No Hit
GGCCAGCAGACATCCTCCAGTGGAACACATATTTGTTGTATAAGATGCAT4410.36676951737788904No Hit
GGTTCCTCTTAGTGACCATATCAGTCATTCTTGTCAGGCAGTGCTTCTAT4390.36510616355758113No Hit
GGAAATGGTAGATGACCAGTGACACTGGTCAGTTATGGGAATCAACAAAC4350.36177945591696536No Hit
TATGTGCCATGGCTGGACTGCCTAGTGTTCACTAAAGATAGTCTGGATCA4280.3559577175458878No Hit
TTTCTTTCTGTCTACGTGGAGGAGCCCTTTGCTCGACACCTTGTTTATTT4180.34764094844434834No Hit
CCTGCCTCAGTACGAGATAAGACACCATGCTCTCTTGTTTTCAGAGTCAA4160.3459775946240405No Hit
CCCCAGTCCCCCTAACATCACATGATTCTGTCAGATCACAGTTGTTCACA4080.33932417934280895No Hit
CTCTTCCCTTCACCCTATAACAGACAGATTCTCTGTATGTAACCCAGCTT4070.33849250243265494No Hit
CAACGGCCTTTCTTTCCTTCATCCTGGGAATTTGAAGCACAGTGAACAGA4070.33849250243265494No Hit
TGGTAGGAGTCTCAGGTGAGGATCCAATGACCTTTCTATGAGTGAAGGTC4030.3351657947920392No Hit
CTTTGGTCTTCGTTCTTCTTGAATTTCATGTGTTTGGCAAGTTGTATCTT3980.33100741024126945No Hit
TGTCCTCCTCCTTTCATAAGTCCCCAACTTCCATCTTGATGCCAAACTCT3900.3243539949600379No Hit
TGCATTGGAGACTCCAAGATGTGAGAGGTGTCAGAGTTGTGGGATATCTT3850.3201956104092682No Hit
TCTGGGTATCATATTTTGTAGTAGCATCTCTACCTTCAGAAGCATTCCAG3820.3177005796788064No Hit
TCCAGTCTACAACAGCTCAAGTGGGACTGCACGACAGATCGGAAGAGCGT3820.3177005796788064No Hit
TTAACAACTTTCATGGAAATGCTGACTGTTGATTTAGCTTCTGTCTCTCC3810.3168689027686524No Hit
TTGGGGGTTTAGCCGGAGCAGCTTTCCCCAGAGCCTGAGAGACAGTGGAT3620.3010670414757275No Hit
CCTGCTATTATAAAAAGTCAATCTCTGGTTTGTTTGAATCTTCCTGACTC3490.29025524164372624No Hit
TGTGGGGTCTGTGACAAGACCTATGTCTCAGAGTTAAAAATGGAGTGCAC3450.28692853400311047No Hit
TCTTCATTTTTCAAGTCGTCAAGTGGATGTTTCACTTTTTCCATGATTTT3420.28443350327264866No Hit
CAAAAGATAGAATGGCAAATAGCTTCTTCCAGTTGTGTTTAACTTCAGAA3370.28027511872187894No Hit
CAGGCTAGTGAGAAGGCATTAGTGGTTAGAAGCCATCTGTAGATGAATCC3170.26364158051880004No Hit
TAATGAAGAGTCATCTGACAGAGTCTCGCTGGGCAACTACACAGTTCAGG3150.2619782266984922No Hit
TCTCATTTTCCGTGATTTTCATTTTTCTCGCCATATTCCAGGTCCTTCAG3120.2594831959680303No Hit
GGAATCTGGACCTAGTGTCATCAAATGAAGCTTAGTACATGGCCCAGGGG3080.25615648832741456No Hit
AAAGGAGCTCTGGGCAGGAAGCCAGGCCTGGGTCACCTACTAACACCGTG3050.25366145759695274No Hit
GAGTAGGACGCGGCGGCGGCCGCGGCGGCGGAGGGACGGGCAGGGATGGG3040.2528297806867988No Hit
CCCCACGGGCCTCTGAGGAGCCGTGGTCACGCGGTTTGCTAAGTTTCTTA2970.24700804231572118No Hit
ACCCATATCTAGAATCCCGGGGACCCACTCAGCAGTAATGTGGGTGGAGA2920.24284965776495146No Hit
GAGAAAGCGCATTTTCTGAGGACTGGCAATGGAGGAAGGAGAGGGCCTTC2890.24035462703448965No Hit
GGCTTTCAGCTGAAGAAATGAGAGGATTTGCTTCAGTTCAGGACCAGCTA2830.235364565573566No Hit
AGGGAGGTGGGCAGAGTTCCAGCCACCAGCTCTGAGAATGCCTCTGCCCC2820.23453288866341204No Hit
CATGTGGCTGTTTGCCTGGGTGAAGATCAAGGCTGCACCTCGCTGTTGCT2740.22787947338218048No Hit
CCAACAGAAGGCAACCGAACAGAAAGGAGAAACCCAAACCACCCCATGCC2730.22704779647202655No Hit
AGGAGGCGTCGAACTTGGAGAGATCCTTCAGCTTTCCAGACCGCTTGGGT2700.22455276574156474No Hit
CGATAGAAAAAACACAGGACAGAGTAGACGGACTTTGTCTCCCTTGGTCT2680.2228894119212568No Hit
TGCCCTGCATGCTGGCAGAAGGCTGCCTTTCATACTCAGGCAGTTCATAA2680.2228894119212568No Hit
GAAAGCAGCACCCCTCCATGCCACCTCCCCTGTATCCTCATAGCCTTTCT2630.21873102737048714No Hit
CTCTAACTTTAGGATAGTACTGAGAATCCTCCTGTCAGAAGACATGAGGA2600.21623599664002527No Hit
GTGGAAGGACTGGAAAATGTTTTCTGGGAAAGAGTTGTAAATGATCAGAG2540.21124593517910162No Hit
GGACAGACATGGTTTGCTAGAGCAGGAACTTACTGTGTTTGAATTTGCTT2510.2087509044486398No Hit
AATGAAGATTTTCAACTTAAAGGGCCAGCAAATATCTTCAACAAAATTAT2470.205424196808024No Hit
TCCCTCTTGGCTGGTGGCAGTCACAGCTGAGTGAGTGGTGAGTCAGCACT2460.20459251989787008No Hit
TGGTCTGGAGTAACTTGTCAGGCAGTGCTTCTATCTACTCTGCTCTAGAA2430.20209748916740822No Hit
CATGCCAGGTGTCAAGTCGAAATTGTTACAAGTTGGGAAATGGGTCAGAA2270.18879065860494515No Hit
TTGTCTTTCTGAGCCTAAACAACCCAAACATGGAAACAGAGGACCTGAGG2270.18879065860494515No Hit
CGCGTGTACCGTGGGGCCAAGCTGCTTTCCCTCATCAGAATGTTTTTTAG2240.1862956278744833No Hit
GCTACCATCGACGGTGCCTTCTCCGCGAAGTTACGGATGAACTTGGCCAT2210.1838005971440215No Hit
GGAAATGGTAGATGACCAGTGACAATGGTCAGTTATGGGAATCAACAAAC2140.1779788587729439No Hit
TCTCAATAGTCACAAATAATATAAAATATCTCAGAGTGACTCTAACTAAG2080.17298879731202024No Hit
TAGGGCATTTTCTTTTCTTTTGTGTTTTTTTCTGAGACGGGTTTCTCTGT2020.16799873585109656No Hit
CAATCAGCCGGTGTGAGGCGGTGCCCACTGTGTTCGCGTCCCTCGGGCAG1930.16051364365971107No Hit
GCCTTACTCTAGTGAACACTAGGCAGTCCAGCCATGGCACATAAGATCGG1930.16051364365971107No Hit
GATTTTATATTTGGTAAAAAATCTTTTCCCATTCTGTAGTCTGCCAAATT1910.1588502898394032No Hit
CTTCAGTATTCCCATTCCTCTGCGGGCTATTTGTGACCACCTTGTTGATT1900.15801861292924924No Hit
TGGTAGGAGTCTCAGGTGAGGAGCAGAAGCAGATTCATTTATTGTAATAG1840.15302855146832559No Hit
TGGCCAGAAAACTGAGCAAAGTAAATAAAAACTTACACATACATGCATGC1830.15219687455817166No Hit
TACCAAGACAGTTTTCCCCGGACACACCCACAGGCCAGTCTGACCTGGGC1820.1513651976480177No Hit
CATAGGCTTTCTAATGAAGATAAAATTTAAAGAGCCATACTAAAAGAAAG1810.15053352073786375No Hit
TTTTTATCTTAGCCATACAGACTGCTGTGAGGTGGAATCTGAGGGTTGTT1770.14720681309724798No Hit
CGGTAAAGAAGGGGGCGGGAAGTCGGTCAGAAAAAGATGGGGCGGGGTCT1760.14637513618709405No Hit
AAGCAAGTCGCCTAGGCCGAGGGTTAGCCACACCTTTTCAGCCATTGGCC1710.14221675163632433No Hit
CCCCATTCTCCACCTTATATTTTGAAATAGGGTCACACACTGAAGCTAAA1570.13057327489416912No Hit
TCTTTTAAAATTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1560.12974159798401516No Hit
ACTACCGAGCCTTGACACTTGAGCAGTGTCATGCGTGTCTGCTGCCGTGC1550.12890992107386123No Hit
TATTGCTTGATTTTTTTTCCTGTAATCCTATAAAATTATTGTATCCAGTG1520.1264148903433994No Hit
GCACCAGAGCTTGAGCCCCTTCGTTGCTAACGGCTCTCAGTAAAGCTCCG1480.12308818270278363No Hit
GATGTGTATCCTGCATGTTCTGTGAAAATGGAGGCTCACATCCCGTTTAC1450.12059315197232179No Hit
TGCCTGGGGTGGAATAGGCAACTGAGCAAGGGTGTCCCAAGAGAAAGGTT1410.11726644433170602No Hit
TGAGTGGTAGCGGCGCTGACGCTAGTCAGGTGGGTCACAGCAGTGCTTGT1410.11726644433170602No Hit
AACTGGAGAGCCATAATGTCAGGGAGAAATTAAGGGGAGGGATGGGTTTT1390.11560309051139814No Hit
GTAATATGGCGAGAAAACTGAAAATCACGAAAAATGAGAAATACACACTT1330.11061302905047447No Hit
TGTTTCATAGCACATAGCTAGTTCCAAACAATCCTGCAGCACACTCTGCT1320.10978135214032053No Hit
TGCAATCCTGAAGCCTAGGCCACTCTGGGGCTCACAGCAATCTTCTCCAC1300.10811799832001263No Hit
GATAGGTTCAACTGGCTGAAAGCCGTGGGTCATCCAATACAAACAGATAA1300.10811799832001263No Hit
CAGTATAATCCCAGGAACGGTGAAAACGAAACCATAGGACACTATGTCCA1260.10479129067939687No Hit
CCACACCAACTGCTCTTAGGTAATGGCTAATCTCAGTTTCCCACAAAAGA1240.10312793685908897No Hit
ACATGGCTGGTCAGCTACAAAGTCCTGTGGCCTCTCCGTGATGCTATTCT1230.10229625994893504No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)