FastQCFastQC Report
Thu 18 May 2023
G202M1_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG202M1_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10611507
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTGCCATGGCTGGACTGCCTAGTGTTCAAGGAAGTCACAGGCCTCTT590600.5565656225831073No Hit
GCAAGGGCTACCATCAGACCAAGAGGGATATCCAAGACAATATCTTGTCA530740.5001551617503528No Hit
ATGTCTGGAGCCGAAAACGGTGCTGCCTCAGAAGCTCTGTGGCTCTCGTC266590.2512272762012031No Hit
GCAAGGGCTGCCATCAGACCAAGAGGGATATCCAAGACAATATCTTGTCA258600.243697714188946No Hit
CCCCTGACCCCTAAGAGCAGTTGGTGTGGAGATCGGAAGAGCGTCGTGTA248500.23417974468659353Illumina Single End PCR Primer 1 (100% over 21bp)
ATGTGCAGCTCAAACATTGGTTCTGTGGTTTGAGTTTCTATAGGTACCAG244370.2302877432960276No Hit
GATTACATTACACATTCTGCAAGCATAGCACAGAGCAATGTTCTACTTTA235880.2222869946747432No Hit
ACTGACCCCTCTCTCAGTTTCTCCATTTTCCTCTCCTAGGAGACACAATC229510.21628407727573473No Hit
CTGGGTGGCCAAAGGATTGTGTCTCATATGTAGGAAATTTTTAAAAAGAA205380.19354461152407476No Hit
GAGCTGGGGGGGAAAAAAACTGAGCTGCCAGATGTCAAAGTAGCCAAAGT196800.18545904931316542No Hit
TCTATACCCTTTCTACAGCCACATACTAAACGTAGACAAGTTGGCCTTTT193860.18268847205208458No Hit
TGAACCCCAATAAGGAAGCTTGAAATATACATTGGAAAAATTGTAGAATT192350.18126548849282198No Hit
AACTATAGCTTTAAAGCCACAGAAAAATATAAAACCAAATAGTGTAGATA189620.17869280960753264No Hit
CCATCTTTAGGGAAGGTATCACATGGGCTTTAAAAAGTGTTTTGGTGTAG188600.17773158892511687No Hit
TGTGAGGAAAGTCGCTGTAGGAACAAAGGCAGAAGCCATGTACCAATGCT184650.1740092147138008No Hit
GGAGGCATTGAGAAAGTCACTGCCCTCCCGCACAGCTCTGGCCAGCAGGG178970.1686565348352501No Hit
GACTCCCTTCGTGACCCATGGATCTCTGCTTTGTATCTGCTTTGTATGAC163870.15442669924262406No Hit
GCACTCATAGGTGGGGAGATGTTGGGGTGGGTCAACTCAAAGCCCTGGAT157930.14882900232737914No Hit
GTGTAGAAGAGCTAGCCCCATGCCTTGCCTGGGCACCCCATTTCAGCTGG157670.14858398529068492No Hit
TAACGTCGTAAGCGGCACATGCACAGTGTGGGGACCGGGGTGGTAAGTTA151400.14267530521348193No Hit
GGCGCACAGGCGCGGCGGGGGAGCGAGGTCGCGCAGGCGCAGACGGCGTT146910.1384440494644163No Hit
AGAAGGAGGATGAGGAGGAGGAGGAAGAAGAGGAGGAAGAAGAAGAAGAA145700.13730377787057013No Hit
ACAACCTCATGTTGGAAGGTGCATTTCCTGAGAAAGTAGGTCATTCAGGG144680.13634255718815433No Hit
ATCTCCCTCTGCCTCCTTCCTCTCACCCACTGTTTTACTTAAAAGCTAAA140990.13286520001353247No Hit
CTCCTGAGCAAACCCTGCAGGACAGGAGACATGACAAATGGTGGGATTGT139050.13103699597050636No Hit
AAACAGACATCTGACACACAAGAAGGAAAAGGCCACGGAGACCAAGCATT138810.1308108263981732No Hit
TGACCCAAATTTCATCTCCAGTGCATGTCTCAAAGTCTAGGAGTAGTGGC137870.1299249955732018No Hit
AGAGAACTTCTGGTGGATTCACCATCCCTGATCTCAAACTGTAATACAGA137460.1295386225537994No Hit
GGTCACCACTCCCCCTGACCTGTGCTGGGGTCCCAGCACCATTGCCTATG134260.12652302825602435No Hit
ACAGGAGAACAGAGCCCTGTTCAGGACCTTCAGTCAAAAAATAGGAAAAG132820.12516601082202555No Hit
TGATGCCTTCTTCTATATAACTTTCCTGGATGTCACTCACACCAATTAAC130390.12287604390215263No Hit
CAAACTTTCAGACAAGGCTGCCTTTTCCACATTTCTTAATTCCTTCAAAA127220.11988872080091924No Hit
GGCTTTCAAGTACAGGACAGCATCAACTAACAGAAGGTCATTAAATGAGG126990.11967197496076665No Hit
GCACTGGTTTTTTACCTCGATGTATTTTCCTTGAGTAACTTCAAAAATGT126830.1195211952458779No Hit
ATGTGCCATGGCTGGACTGCCTAGTGTTCAAGGAAGTCACAGGCCTCTTC126740.11943638165625298No Hit
TGCTTAGTTTTCAGGTTTTGTCTATGGATTCTGGTGGGGTCGGGCAAGGG126420.11913482222647546No Hit
ACTCCATTGAAATGGCAAAATTATCATTTTTTGGTTGTTGTTTTGTGTGT125570.11833380499112897No Hit
CATGGCCAAGCCAGATGGTCATGAGCATCAGAGAGGTCCAAGGGCCAGGC124970.11776838106029613No Hit
CACAGTTGGCACAGCACAAAAGTCACTATATCTTTGGATAAAGTGAATTC124290.11712756727201895No Hit
CAAACTTTCAGACAAAGCTGCCTTTTCCACATTTCTTAATTCCTTCAAAA123180.11608153299997823No Hit
ACTACTGAGTCACTCCTCCAGAGTCTCAAACAATGCTCTTTAAACCCCAC121640.11463027824417399No Hit
ATTACATTACACATTCTGCAAGCATAGCACAGAGCAATGTTCTACTTTAA121360.11436641374311866No Hit
CTCCTGTAGCCTTGGCATCCCATAGCAGGAATGCTACAAAGTTGTCCTTG121100.11412139670642445No Hit
CAGCAGGAGGACCAGAACAGAGAGAGTTGCTTGGTACCTGTCCACAAGAT120780.11381983727664693No Hit
TGAGTAAAGTTTAAAAACAGTGACCTCTTATTAAAAGGTATGATTTCTTC120480.11353712531123054No Hit
TCATTTCCGAATATTCCCTCTGTCTACTTATTTACCCAGCGTATCTCAAG119920.1130093963091199No Hit
TGTGAGTGGGGAGAAGGGAAGTGGTAATAGGGGAGGCATTTGTGAGGATG117040.11029536144112236No Hit
TGTGTAAAATCATCTAGAATCATCACAGTGGTCTCACTCCATACACAACC115570.10891007281058196No Hit
CCTCCCTAGTCAGGATTGGCACGACCCCTAGGGCGTTGTATAAGTTCCCA114490.10789230973508286No Hit
TGTCTGGAGCCGAAAACGGTGCTGCCTCAGAAGCTCTGTGGCTCTCGTCT112630.10613949554950113No Hit
TCCTGTAGCCTTGGCATCCAGTAGCATGAATGCTCCAAATTTGTCCCTGC112330.10585678358408471No Hit
GAAACCTACATCACAGAAGGTGAGAACCAACTCCCGCAAGCTGTCCTCCG112060.10560234281520994No Hit
CCTGACACCACGGCAGATAATGAATACTGATTGGCTTTGTGATTTATGAC111340.10492383409821054No Hit
TTTTCTTCCCCTGACTCCTTTTATATACGTGTAGTTTTCCTGTCTCATTC111040.10464112213279414No Hit
GAACACCCTGAGGCCCAAGGGCCTCTCCTCACACTGATGTGAAACAAGCC109700.10337834202060084No Hit
TAGGGTCCCCAAAACAAAAAGAGGTATTAAACTCAGTAGGCTGAACCTTT108850.10257732478525435No Hit
GTTGATTATTAATCTTTGGCCGATGGTAACACTTTTGTGTTCAGAATGCA108840.1025679010530738No Hit
TGGTAGAAGGGTCCAAACAGTTAAAAACAGAAAAGAAAAGAAAAAGTTGC108090.10186112113953277No Hit
TCCCTCTCTGCCATGTTGGGAAGGCCCATGTTACACAGAACTGAGGGTGG106810.10065488342042275No Hit
GGAGGCGAAACTGAAGCTTGAAAGATGCTAAAGATTTACCATTTGACTAG106660.10051352743771455No Hit
TCTCTATCTTGGGTGGGGCAGAGGAGAGAGGCTTGCACTTCTTCTCCAAG106620.10047583250899236No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)