FastQCFastQC Report
Thu 18 May 2023
G202M2_CKDL230013148-1A_H5NKYDSX7_L1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG202M2_CKDL230013148-1A_H5NKYDSX7_L1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13979996
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCAATGATGTGTATTGGCAAGCTCATGCTAGTGATTAATTGCTGGGAA444540.31798292360026426No Hit
GCACTCATAGGTGGGGTAGATGTTGGGGTGGGTCAACTCAAAGCCCTGGA374310.2677468577244228No Hit
ACAGGTATCCACAACTGGCTCTAGAGTAGATCCAAGTACACCTTCATCTC333180.23832624844813974No Hit
GTCTAGGCCTGTCTTTCCATTTGCTAAAAGGTTGCCACGACAACGTCTTG268930.19236772313811823No Hit
TTTTTCTTGGATTTGAGCTTGCATATGACTTGTCTGTGTTGTCTTTTCTT254080.1817454025022611No Hit
TTCTCAATCTTGAAAGGGCACCATAAATGTTGTACAGGAGAAAGATCGGG214400.15336198951702132No Hit
TAGTATTTCCTTGTGTAAATATGTCACATTCTCTATTTACTGAAGGGCAT206510.14771821107817198No Hit
CCTCCCACCCCTGGGCTGTACCTGCCACAACCTTCTCTGCGTGACCTGTG204140.1460229316231564No Hit
TACAAAACAACTCTGGCATGGCGTCGCTGTTAGCAACCGGAGCTGTCAGC194190.13890561914323868No Hit
GGGAGGCCGCCTTTTGTTTGCAGGATCCAAATTAAGACTCAGCTTCTCTC193070.1381044744218811No Hit
GTGTAGAAGAGCTAGCCCCATGCCTTGCCTGGGCATCCCATTTCAGCTGG191640.13708158428657632No Hit
AAGTCCAGCAATATTTCAAATCTAAGTTTGCTCTGAGTCAACTACAGACC190080.13596570413897113No Hit
TTTCCTCTCTATGTTTGACTCTTGGGAGAGGACTATATGGTCTGCTTACC187090.13382693385606118No Hit
TCATGGAAGGTCAGCTTTGAGGTTTCAGAAGCCCATACCATTCCCAGTTA181050.12950647482302569No Hit
GGCTCTCTTGTCTACAGCACAAAAATACAGTGTATCTGTAAATAACTTTC176900.12653794750728112No Hit
GCCTTACTCTGAGGAATCAAAGCATAAGGCTGAAATGTTTTATACAATTA176150.1260014666670863No Hit
CTGCTCTGCCGGCTGGAGGACAGCCATCCTTATTGAGTGTATTTCCAAGA165330.1182618364125426No Hit
TGACTTAGGAGGAAGGTCAGAAAAACATGGTGATTTCGGGCACAGAATCT164490.11766097787152444No Hit
CTCCCCCTCTGTGTAGAATAACACCGTGAGCAAATGCCTTGTGTCTTCAG162180.11600861688372444No Hit
TCTGGGGTATCAGAGTTTGCATTTAGTTCCAAGACCCTGGGGTCCTCTGT162000.11587986148207767No Hit
AGACAAAGTGAAGGAGCCGATCATTTACCAGATCGGCCGCGAATACAACA157620.11274681337534002No Hit
TTAGGTATAGCAGCAAGTATCTTCACCCTCAGAGTCACTACTCCCACACT156710.11209588328923699No Hit
GACTCCCTTCGTGACCCATGCTAAGGCGAGTTAGCCTGAGATGGTCTCTC155470.11120890163344825No Hit
ATGTCTGGAGCCGAAAACGGTGCTGCCTCAGAAGCTCTGTGGCTCTCGTC154760.1107010331047305No Hit
CTGGACATGGCCCTGAGACTCAACGCTGGTCCTCACTTCAACAAAATGAG153690.10993565377271926No Hit
CAGGTCCCCCTTTGGATGTCAGCGGCGCCCCTGTTTACCCGCCCGGCTGC152270.10891991671528375No Hit
AACCACGCCTTCGCCCACTTTCCAGCCCCGCCCCTCTCGGGCCGCCCTGA146130.10452792690355563No Hit
CATCCTGGGAGGTGTGGACTAAATTACTGACTATCAAAGAGTTCCAGGAA144570.10341204675595042No Hit
AGGGATAGGAGTCCTTTGTGTTTGCTGGCTTGGCTGTGCCCTCCACCTCT144550.1033977406002119No Hit
TGAGATTCTCTCTTGCATGACCACTCAGGCTTCCCTGTAATCCCGCTAAA143790.10285410668214783No Hit
GCGGTTGACCTGCAGCAGTGATCTGCTGGGGACAAAGTTGTCTCCCCCTC143140.10238915662064568No Hit
TCTATACCCTTTCTACAGCCACATACTAAACGTAGACAAGTTGGCCTTTT142410.10186698193618939No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)