FastQCFastQC Report
Thu 18 May 2023
G202M2_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG202M2_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13979996
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGGAGTATTAAGTCAGCTTCCACTGGCCTCTCTGAGCCAGCATACC442290.3163734810796799No Hit
GTGTAGAAGAGCTAGCCCCATGCCTTGCCTGGGCATCCCATTTCAGCTGG361180.2583548664820791No Hit
TGTCTGGAGCCGAAAACGGTGCTGCCTCAGAAGCTCTGTGGCTCTCGTCT339450.24281122827216833No Hit
AACTATAGCTTTAAAGCCACAGAAAAATATAAAACCAAATAGTGTAGATA302460.21635199323376061No Hit
GACTCTTTGTTAGGGACTTTCACACTCCTAATAGAAGCAAAACTGCATTC280410.20057945653203335No Hit
ATTTTGGTCAAATTCAACCCACATCTCAGTTGAGCAATAGTGGAACAAAG253660.181444973231752No Hit
TATGTGCCATGGCTGGACTGCCTAGTGTTCAAGGAAGTCACAGGCCTCTT251110.17962093837508966No Hit
CAGCTTCTAAAAGCAGGAGCGGTGTGTCCAGATTGGCCAGCATTTTCTCT214270.1532689995047209No Hit
AAGACACCTCTTGTTCATTTTCCCTATCAGAAGCTTTCCAACCAGAGAGG206060.1473963225740551No Hit
GCACAATCTCTGCCTCAGTGACATGGCCAGAGGGGCTGGTGACATGGGCA201200.14391992672959278No Hit
GTGTAGGAGGTTTAGCAAGAGCTTTAAATCCAATAGAGAAGGAATCAGGA197260.14110161404910274No Hit
AGTAAGCACTTTATGTGTAAACCATGCTGGCTCCTTTTCAATCATCCTGT193910.13870533296289927No Hit
GCACTCATAGGTGGGGTAGATGTTGGGGTGGGTCAACTCAAAGCCCTGGA185730.1328541152658413No Hit
GAGCGGGCGGGGTGGGGGGAGGAACAGAAACAGAAACATTGACAGAAACA177800.12718172451551488No Hit
GGGTTCCCACCTGGCCAGAGCCAGGAGGAGCATCGAGCAGGAGGCGGGCC169510.12125182296189499No Hit
TCACATTGAGTTAGAGCCAGCTAAGATAAAGAGACTCCCAAACAAACATG160780.11500718598202746No Hit
AGGGTAATCTGAGTCCAAGGCCATTCTGGTCTGCAGAGTGCATTCCAGGA157470.11263951720730106No Hit
CAGAGAGGTCCATGGAATCCATTACCCCGCAGGCTGACAAGAAAGAAGGA156950.11226755715809933No Hit
AGGCTGTTTGCTTAAGGGATCTAAGGGATCAAGCCTACTGTCTGAGGCTC156440.11190275018676686No Hit
AGAAGGAGGATGAGGAGGAGGAGGAAGAAGAGGAGGAAGAAGAAGAAGAA149230.10674538104302748No Hit
TTTCCTCTCTATGTTTGACTCTTGGGAGAGGACTATATGGTCTGCTTACC146980.10513593852244307No Hit
CTAGAGGCTGAAGGGATCCCTATTATTGTCCAACTTCAGACAAAGCATAA145960.10440632457977812No Hit
ATGCCTGTTTACATGTGCAAAGTTCTAAAGAGGCAAAGAGAAAGCAAACA144220.1031616890305262No Hit
ATGCACTTTGCTGCCAGTCGCGGCCACACCAAAGTGCTCAGCTGGCTCCT144080.1030615459403565No Hit
GAAACCTGAGGGAGAAAATGGACTTCACACTCCTAACAGAATCAGAGATC143910.102939943616579No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141650.10132334801812534No Hit
GCAAGGGCTACCATCAGACCAAGAGGGATATCCAAGACAATATCTTGTCA140130.10023608018199719No Hit
GATTACATTACACATTCTGCAAGCATAGCACAGAGCAATGTTCTACTTTA140050.10017885555904309No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)