FastQCFastQC Report
Thu 18 May 2023
G202M4_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG202M4_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9250365
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTATAGCTTTAAAGCCACAGAAAAATATAAAACCAAATAGTGTAGATA185190.20019750572004458No Hit
CTCCTGAGCAAACCCTGCAGGACAGGAGACATGACAAATGGTGGGATTGT162150.17529038043363696No Hit
CTGGAATCAGACGCCCACAGGAGCCAGGCAGGACACACAGGGCCTGTGCT151380.16364759660835004No Hit
AGAAGGAGGATGAGGAGGAGGAGGAAGAAGAGGAGGAAGAAGAAGAAGAA148390.16041529172092128No Hit
GGGCAATGTCACAGGAGAGGCAGTCTGTGTATTTGAGGAGTGTCAGGAAA139010.1502751513048404No Hit
AAACAGACATCTGACACACAAGAAGGAAAAGGCCACGGAGACCAAGCATT126000.13621084141004167No Hit
CACCAGCAGTTTAGTGTCAACAAACACTGAGCAACATAGCAAACACCAGG119270.12893545281726723No Hit
GCCAAGCTGCACCCCCATGACCTACTTAATCCAACCAGGCCAAGCCCCAC114020.1232600010918488No Hit
ATGGCTCCCACTGGGAATCCTGGGCTGGAATACTGACCCAAGTTCAGTGC112020.12109792424407036No Hit
ACCAGCCCACAGCTGAGTGTGCCTCCCAGTGAACCCTGAAGCTGGGCTGT107400.11610352672570218No Hit
GAGCAGAATCAGAGACAGCGACTGCCACAAGAAGAGATAAAGTGGAGCTT107310.11600623326755213No Hit
CTGTTGACAGACAGCACAGAGTGCCATGGGTCAGAGAAATTGGCAGTCAC107180.11586569827244655No Hit
ACCAGAGAGGACTGCAGGAAAGCTTGGGAAGTGGCGGGGTAAAACAAACA103390.11176856264590641No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)