FastQCFastQC Report
Thu 18 May 2023
G202M7_CKDL230013148-1A_H5NKYDSX7_L1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG202M7_CKDL230013148-1A_H5NKYDSX7_L1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12516261
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCCAGCAATATTTCAAATCTAAGTTTGCTCTGAGTCAACTACAGACC438940.35069578686478337No Hit
TCATGGAAGGTCAGCTTTGAGGTTTCAGAAGCCCATACCATTCCCAGTTA426330.3406208930925937No Hit
ACAGGTATCCACAACTGGCTCTAGAGTAGATCCAAGTACACCTTCATCTC297730.23787455375051703No Hit
AGGTCAGCTTTGAGGTTTCAGAAGCCCATACCATTCCCAGTTAGCTCTCT295400.23601297544050895No Hit
GGCTGGCGTCTGAAGCCCCAGCCTAGCAGCATCGGACCTGAGTTCCTCTG288820.23075581437619427No Hit
AGGCTATCACTGGCTTCCCTTTTGTCTACATAAATGCTTTCACGGGCCTG272890.21802837125240518No Hit
CCTCCCACCCCTGGGCTGTACCTGCCACAACCTTCTCTGCGTGACCTGTG232480.18574237146381015No Hit
CGGCTTGCTTGCTTGCTCGCTTTCAGATCTCTAAAGGCATCTTGGTTTCT206960.16535289572500925No Hit
TGATGCTTTACCTTCATCACTGATGCGAACATCCAGCTTCTCTCTGACTC199840.15966429591073564No Hit
CCAACACAAAACCCCAAGAAAAATCATTGGTTCACCAAGTTGGGCTTTGG193910.15492645926766788No Hit
TTCCTGTGATTTTTTTTTCTCTTCCAGAGTTCAAAAACTGGTGACGAAAG177910.1421430888985137No Hit
ATGTGTGACCCCTCAAAGGGAGGAGATTCCGTGGCGCGGTGGCAGTGGCC177000.14141603470876807No Hit
TCAATCAGTGGCATCAATTTGAACAAATTATTGAGAAATGATAAAGCTGT173500.13861967244051557No Hit
CTCCTGCCTGATGGAGTATCGATTCTCCGAGTGCAAAGTCCAGCATGCCG173330.13848384913034334No Hit
TTAGCTCCAGACTGACACAAATGGTTTGAAGCCTGTTGTTTGCTGCTTCT166570.13308287514937567No Hit
GCCATTGATTGACATGAGGCACTGGGCCAGACAGATGAATTTCTCTGGTT162710.12999888704781723No Hit
ATGCTTTCCTTTATTTGAGTTTCTGTGGTCATGGTGCCACTTCATACCAC161000.12863266433961387No Hit
ATAATTACCAGGGCTTCACCTTTTGGCCACTATACTGTTAATTTGCAGTT156170.12477368440942548No Hit
AGAGCAGACTGGGGGCTGGGGATCTGATTATTGACAGGGGAGGAGAGAAC155060.12388683809006538No Hit
GGTTGATTTCTCTGACCCTCAACTGGCCTGATTAGAAGACCTGGCATTCT147770.11806241496561953No Hit
TTAGGTATAGCAGCAAGTATCTTCACCCTCAGAGTCACTACTCCCACACT143650.11477069709556233No Hit
GAGCTATTTACTTAAACCTTCAGTATCCTGGCCTTTGTCCCCTCTGCAGA141110.1127413370494591No Hit
TTGGTGTGTGTTGACTACAAATCACTTTGTTCTTTTATGTTTATCAGCAT137440.10980915147103436No Hit
TCACTGGTGATGAGAAACATTGCATGGAGGGCTTCAGAGTCAGAGGCCTT136990.1094496191794019No Hit
ATCCTATGCTCACTGAGGATTGCTGGTTATGTTTGGGCTTGGGGGTGACG136760.1092658582303453No Hit
CCCTGAAGGTCCGGAGACACATGGGAACTCACCCCTCTGCCCTCCGCCCT136530.10908209728128873No Hit
TTATGGAAAAAGTCAACAACAGGAGTCAGAGCAGCCGGAAAAGCATGGAC135870.1085547832535611No Hit
TATAAAATCAAGGATTGATCAAGGCTGTGGACTTTGGTTCTTATCACTTG133070.10631769343895912No Hit
CTTACGGAGAGTGTAATTTCTTTCCTGCTCCTATGGAAATTTTTTTGTCA132170.10559862885569421No Hit
TGAGCCTTGCAAATATTAATGAATACTGCTTTGCACTCCCAGCTTCCCCT131700.10522311735110029No Hit
CTGCTTGCCCACTTCTTTATTCCTCTGTCACCCTAAATTTCAGGTCAGAG130850.10454400080023898No Hit
AAAGCAAAGGTACTGAGTCCTGTGTGTCATCATTTCTAAGAGCTCCCTGG129380.10336952864757294No Hit
TCCTACCTGGAAGTGGCCCTGAGAAAGTCTTACCCAACCCAACCTGGGCC127790.10209918121713824No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)