FastQCFastQC Report
Thu 18 May 2023
G202M7_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG202M7_CKDL230013148-1A_H5NKYDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12516261
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGAGGGCAGAGGGTAAGGGCAGAGGGTAAAGGGTAGCAGGAGGCAG406640.32488935793205337No Hit
AGAAGGAGGATGAGGAGGAGGAGGAAGAAGAGGAGGAAGAAGAAGAAGAA384450.3071604211513327No Hit
AACTATAGCTTTAAAGCCACAGAAAAATATAAAACCAAATAGTGTAGATA303130.24218894125010654No Hit
GGCCAGTGGGCAGAGGGCAGAGGGTAAGGGCAGAGGGTAAAGGGTAGCAG298060.23813821076438085No Hit
CTGGAATCAGACGCCCACAGGAGCCAGGCAGGACACACAGGGCCTGTGCT281170.22464376541844244No Hit
CTCCTGAGCAAACCCTGCAGGACAGGAGACATGACAAATGGTGGGATTGT279810.22355717893706434No Hit
GCACAATCTCTGCCTCAGTGACATGGCCAGAGGGGCTGGTGACATGGGCA229770.18357718810753468No Hit
AAACAGACATCTGACACACAAGAAGGAAAAGGCCACGGAGACCAAGCATT212820.17003480512271196No Hit
GAGAGATAGGAGTGCCCAGCCAGCCACAAAGGCAGAAGAAAAAACACACG204780.163611161512212No Hit
TAGGGAAGTATGACATTCCTGGCTGCAGACCAACTCCACATAACCCTGGA186250.1488064207034353No Hit
GGGCAATGTCACAGGAGAGGCAGTCTGTGTATTTGAGGAGTGTCAGGAAA178680.14275828859752923No Hit
TTGCAACTCCAACCCCAGAGGATCTGACACCTCTTATGGTGTCATTCAGA174280.13924286174601183No Hit
CTGTTGACAGACAGCACAGAGTGCCATGGGTCAGAGAAATTGGCAGTCAC164050.1310694943162339No Hit
TGAGAGACATCTATCCTGGGGAAACACAAATAAACATTTACTAACCCCAG163380.13053419068202557No Hit
AGGGTAATCTGAGTCCAAGGCCATTCTGGTCTGCAGAGTGCATTCCAGGA161410.12896023820532346No Hit
ACCAGAGAGGACTGCAGGAAAGCTTGGGAAGTGGCGGGGTAAAACAAACA159040.1270667014693925No Hit
CATTCAACTCATGAACATGAGATCTATGTGGGACAACACACAAGATAGCA157240.12562857230286265No Hit
CCCGCATCATGAGACCCCAGTCAGCACAGGCTTCCAGGTACGGTAAAGGG154660.12356725383083655No Hit
GCCAAGCTGCACCCCCATGACCTACTTAATCCAACCAGGCCAAGCCCCAC152080.12150593535881042No Hit
GGGCTGCCAGGGTCCAAGTCCAAGACTTCTCTAACCCCACCCTGTAGCTC147050.11748716329900757No Hit
ACCTAGTGGGTCCACATCAACAAGCCAGCAGGGTGGGCTGGATCAGCACA143120.11434724795208409No Hit
GAGCAGAATCAGAGACAGCGACTGCCACAAGAAGAGATAAAGTGGAGCTT138920.11099161323018113No Hit
TTAATCTCTGTCCAGGTCAGTTGGCTGAGGCAGGAGAGCAAACAGGGCAA136040.10869060656373337No Hit
GGTGAAAAACAGCTTCTTAAATTGGCAAGGTATGCAAAGCTATTGATTCA134890.1077718018184504No Hit
CACCAGCAGTTTAGTGTCAACAAACACTGAGCAACATAGCAAACACCAGG133900.10698083077685899No Hit
GAGCTGGGGGGGAAAAAAACTGAGCTGCCAGATGTCAAAGTAGCCAAAGT131240.10485559545298712No Hit
TAGAGTTCAAGGGTACTGAGCCCAGAGAGCCCTTTAAAGGCTGAGAGTCA130860.10455199040671971No Hit
ACACTGAATTATTTAGTGGTGAAAGGTCATGATCTTTGTAACTTGCCCTG128610.1027543289485574No Hit
TAGGGTCCCCAAAACAAAAAGAGGTATTAAACTCAGTAGGCTGAACCTTT127880.10217108767546475No Hit
GTTGCATTGACCAATCATATTTTGACACAAGGGATTAAATGAGGAGGTTA127700.10202727475881175No Hit
TGTCGCTGCAGCGGCAGTACCAGACACTTGAAATGTCACCGGTCGGGATA127560.10191542026808166No Hit
CCCGTTTTCTAAGCCAAACTGAATTCACATTATCAGATATTTTCAAACTA125490.10026157172657232No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)