FastQCFastQC Report
Thu 4 Jan 2024
G219M18_CKDL230043734-1A_22GGGVLT3_L8_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG219M18_CKDL230043734-1A_22GGGVLT3_L8_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18960963
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTCTCTCAGGAACCTCTCCTCACTGATTCACCTCTTGAAGCCTTC1552520.8187980747602323No Hit
GCTGCCTCTGCCGCCGCCTCCCCTGGGCTCCGGCCTCTTCCAGGCCGCGC965460.5091829987749039No Hit
TCCTGCTGTGTCACTTGGGATGACCAAAATGCTATCAGGCTTCTGCTGTA819370.4321352243554296No Hit
TGCACCATTGCCGGGCTCTGGCCACCCTAGGTATTATTTACCACGGTCTC570180.3007125745670196No Hit
ATTTCTGTCAGGCCCTGGGCCCTTCTGTAGCCACCACAGCCTAGCCTTGT556560.2935293951050904No Hit
CTTGACCCAGGCTCCCAGGCAGTTGAGGCCGGAGCCCAGCAGCGCCGTGA556320.293402819255541No Hit
GATACTGAGATGGCCCTGTGAGCCCTCTCGAACACACAGGGTTGTAAATA434540.229176123596676No Hit
ATTCGACTGACTGGCTTCCTGCTCTGTCTGCATCTGTGGTTTCTGCCACA431850.2277574192829763No Hit
CCTCTGAGCCATCTCTCCAGCCCAGGGGGCCAGGGGTGGGGGGTGTGTTT421030.22205095806579023No Hit
ATCATGGAGAGTCCAAGAGCCAAACCCGGAGGTGAGCAGAGTCCAAGAGC416850.21984642868613796No Hit
ATCGGGCGCGGCGTTTTGGGGGCGATGCGGGGGCGTTTGGCGCGCGAAGC413580.21812183273602717No Hit
CAGTGTAGAGAATTTGACACTTAACTGTCCACAGGGCTTCTGTCAGTTCT412210.21739929559484927No Hit
CACTTGAACGTTGCCATTTCCTTCAGTTTGCTCTGTAACTCATCCTTGGC357730.18866657774713236No Hit
CTGGGCCTGTGGATGCTACTGCGCTTTTTTAGAGAGAGGCAAGTGGTGGA357200.1883870560793774No Hit
GCTTCCCAGTCTGTGTTCTCCCACTTGGGGGGTAGAGGAACGGCAGACTT295210.15569356893951009No Hit
CCTTGCTTACTGAGTTTTGTTTGTCCTTTTCTTTTGCAATAGTGTGCTCG282520.14900087089458483No Hit
CCTCATCCTGGATGCTGCTCTTGCCATGGAGTTGCTGTGTACCTCTTTGC264530.13951295617211004No Hit
GCAGCAGGAGCAGCAGGCTCCTGTCTCTGGCAGGTCTTGAAGGATGCTGG259550.1368865072939597No Hit
GGCTGGATGCTGCTTGTTGACTTGCTCCACATGAGCAGTGCCAAGCCTCA259060.13662808160112963No Hit
CCACTCTTCCCTTCCCTTTGGGGCCTGAAGCCTTCTTGGTCTAATCTGTT251540.132662038315248No Hit
TATGAACTGTTCTTGGAAAAATATCCCCAGTTGCTTCATAGCATCCCTAA243980.1286748990544415No Hit
AAAATCCCTTGACCTGCATTGTTTTTCCTCTTCAGAAATAGGTTAAATGG239340.12622776596315283No Hit
TTGTACAAACAGCCTTTGGAATGAGCCTGTTTGCTTGCTATGGGAGGCAG238900.1259957102389789No Hit
CCGGTCAGGTTCAGGCATTGACTACACAAGTACCCAGGCTGGACCGGCAG236760.12486707558049663No Hit
GAGGTGGCTGGTGCGGAGCTCCCGCCGCGTCTGTGCCTGGTCAGTTTGCC228830.12068479855163473No Hit
GCGGAAAGGAGGAAGGAACAATCTCTTGGGAATTGAATAAGGTTTTGTAC228770.12065315458924739No Hit
TGTTGTCTGTCGCCCTGTAGAGGATCCTGGCTCTGGGCCCTGTTCTGCAC228050.12027342704059915No Hit
CACTCCAAGCAAGTCCCCCAGGGGCTGGGCATTAGAGCAGAAGTCACAGA226350.1193768481062908No Hit
AAAAAGCTGTGGGATCTCCATGACACAGGGTATCCAAGTAGAGTCCCAGT223820.11804252769229073No Hit
CCAGGGCTCTTCAATCTCGCTCTGATGGAGTCGCTGATTCACAGAGGGAA222040.11710375680813259No Hit
TGGATTTCTGCTGAAGTCTCCTGCTGGAACGAGAACAGCACTATTGCGTT214290.11301641166643277No Hit
GAGGTGAGAGGCAGGCGAGCTGACGCTGGCTGTTTGGCTGCCAAACAAAA206970.10915584825517564No Hit
TGGCCCTCCCAGTTGCAGGAGGCCAATGCCCAGCTGGCCGAGCAGACCTG199920.10543768267466161No Hit
AAGCACTCAGAAATCAGAAGACGATGTTGGATCCACTAGAACAGGAGTTC196090.10341774307560223No Hit
TTGCACATGGCCACTCCAGCCTGGCTGGATTGCTGGATCATCTCTCCTCT193570.10208869665533338No Hit
AGGCTGGACCGGCAGTTGATGGCAGAGATTCAGGCATCATGGAGAGTCCA192360.10145054341385508No Hit
TCCCATCATGTTCCTCAGGGCTAGAATGAGGATCTGAGCCGACCAGTTAA189690.10004238708761785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)