FastQCFastQC Report
Thu 4 Jan 2024
G219M22_CKDL230043734-1A_22GGGVLT3_L8_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG219M22_CKDL230043734-1A_22GGGVLT3_L8_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18542810
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATGGAGAGTCCAAGAGCCAAACCCGGAGGTGAGCAGAGTCCAAGAGC820740.4426189989543116No Hit
AATGAGCACAGAGACTAAGGGAGAGCCAGTGGACAGTGGTTGTCCCCTGA555090.29935592286174534No Hit
CTCCTTGGGAGGGATGAGATCTGCTGCCTTTTAAGAAGCTCTCAGGGGTG521680.2813381574853002No Hit
CTCCGCACCGCGAGCACCCGCCCAAGAAGGGCTATGATTTCGACACGGTC486460.2623442725239594No Hit
GGCTGGATGCTGCTTGTTGACTTGCTCCACATGAGCAGTGCCAAGCCTCA457250.24659153601854306No Hit
AACTGCTTTTCAAGGTGGTTAATGATTTCTAGAGGAGGCAAAATAGGGAT444980.2399744159596091No Hit
CCACCCTGTCCCAACCCCCATGCCCTGTTCCTGCAGAGCCTGGCTCAAAG383410.20677017129550482No Hit
GCTGCCTCTGCCGCCGCCTCCCCTGGGCTCCGGCCTCTTCCAGGCCGCGC374540.2019866460369275No Hit
TCCCATCATGTTCCTCAGGGCTAGAATGAGGATCTGAGCCGACCAGTTAA370480.19979711812826642No Hit
CCTCATCCTGGATGCTGCTCTTGCCATGGAGTTGCTGTGTACCTCTTTGC366480.19763994777490576No Hit
AGGCTGGACCGGCAGTTGATGGCAGAGATTCAGGCATCATGGAGAGTCCA343930.1854788999078349No Hit
CCTTTTGTGATGTTCAGCTAATGCCTGTCTGGATCAGGACACAATCTTGA313210.16891183159402484No Hit
CAGTGCCAAGCCTCAGTTGCCCTCCAAGACCTTTATACCTTTAAAATCAC304760.1643548092225504No Hit
AGGCAGCAGAGGTAGAGCTTCCTTGTTGCCACAAATGAGAACACAAGAAT301590.16264525171751207No Hit
GAAGTCATGGTTGTTTGGATAGTTCAGGCCAAACAGGGCCATTTAGTTGG274790.1481922103499955No Hit
GTCCCTAGATGTTGCTTCTCATACCCTGGCTCCTGGGGACACATCACCAC272360.1468817293603289No Hit
TTGGACTTTCTGCCTGTTTGAACCAATGAATAATTCTTGTTGAAAGTGAT271880.1466228689179256No Hit
CCACTCTTCCCTTCCCTTTGGGGCCTGAAGCCTTCTTGGTCTAATCTGTT263780.14225459895237022No Hit
TACCTCCCAGCCTCAGTCTCTGTGCCTCTCTGGAGCCGGTCAGGTTCAGG259250.13981160352718924No Hit
AACTGAGCTCCTTGTCCCTAATTGTACCTGGTTTTAAATTTCCAGCCTTT255710.13790250776446503No Hit
GCTTCCCAGTCTGTGTTCTCCCACTTGGGGGGTAGAGGAACGGCAGACTT254730.13737400102789166No Hit
AGGACACAATCTTGAAGAGGTGCACTCCAGTGACTTAGGAGGAAGGTCAG249080.1343269979037697No Hit
CCAGGAATTGAACTCAAAGGGTGTCAGAAAGCAGGTGGCCTTTGTGCACC236280.12742405277301552No Hit
CACGCCACGCTGGAGCAATGGAGCAGGTCTTCCCCTAATGAGCTGCACAC228460.12320678473219537No Hit
CCGGTCAGGTTCAGGCATTGACTACACAAGTACCCAGGCTGGACCGGCAG225070.1213785828577222No Hit
TGTGTACCTCTTTGCCTCTAAAGGACATCAGCAGGGCCAGCTTGTGCTGT224200.12090939830586625No Hit
AGACAGGACACCCTCCAGCTCTGGTTCTTGATGACCTCAGCAGCTTCCCC223300.12042403497636012No Hit
TCCCCTGAGATGTGATCCTGAGACAGAATCCCAGGGCAAAGTCCTGCAAG221980.11971216875975108No Hit
GGCTGGGAAAGTCTGAAACTGCACCCAGATGGCCTTGCTGTTAGGCCTTA221810.11962048901973325No Hit
CCAAGACCACATGATCTTCTCAAGCAAGAGAGGACAGAGATCTCATGCTC218920.11806193343943017No Hit
TCAAAAGCAAAAATCAATCTCCAACTGTCCAAAGTCTGGGATCCAACTCA214040.11543018560833011No Hit
TTACATTTCTCTTTTTGTTTTCCAGGCTACCCTGAGAAAAAAAGACATGA207120.11169828089701615No Hit
GCTAACATGGCTTTCTGTAATTGAATGCAATTGTATTTTCTATGCTTGTC201220.10851645462580915No Hit
GAAGAAACAACACACTTGAATCCGTCATGGGTGGCAAAGCCAGCAGGCAC196110.10576066949939088No Hit
CCTTGAGGTCAAGGACTTTAGCCTGAGTCTGAGTTGGAGTTTTGTCAACC195800.10559348879700542No Hit
TTGGACCACGAACAACAAGATTTTTCCCTTCTGTTAACAGATCAAAGACA194610.10495173061688061No Hit
GAAGGAGCTAGGTGAGGTGCCTGACACCTGGCAGGATTTTGCCCAGTGGT193430.10431536536263922No Hit
GGAAGGGCTTTTCAGTCAGAAGGGCTGGCGTGGCTGGCGTGGCTGACTGG190870.10293477633648838No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)