FastQCFastQC Report
Tue 22 Oct 2024
G187M25.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG187M25.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19895228
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTGGCAGCTGCAGTAAATGAACAACGTGGGTGTCACACTGGAGGGTCC12516776.291342828541597No Hit
GTGACTGTACCAATACCATGCAGTTTTTATCATGATTCCTATGTAGTACA8050044.046216509808281No Hit
AAACCACACAAAGACTCAACAAAGAAAGAATTTAGGAACACATCAAAATG1494080.7509740526723293No Hit
CCTTTTTCAGTTTTCCTCGACATATTTCACGTCCTAAAGTGTGTATTTCT531440.2671193313291006No Hit
TGAGGGGAATCAATAACTGGAAACTCACTATTTGAGTACAGAGAACTGGC463290.23286488599175642No Hit
GGAAGGAAAGGGGAAGAGTCATAGAAAGGTATTTCTTTGGTCATTGCACC459350.23088451160248077No Hit
TAAAATTATTGTTATATAACCTATGTTTTTATTATCTCTAAGTGTGTGTG452100.22724042167297603No Hit
AGCTACACCCTGGAAAGACCCAACAAAGAAAGAGAACGTCAGTCTGATTT391400.19673059288388148No Hit
GGAATCACATCTCCCACTCCAGATGAGTCCCTATAAAACCTGAACTCTTG352480.1771681128761128No Hit
CCAGCAGCATCAAGAGTCGTTTTTCTGATGCCTTCTTCTATATAACTTTC291170.14635167789984616No Hit
TGTGAGTGGGGAGAAGGGAATGTCTTGTATTAGTTTATTACAGGGAGGGC228760.1149823465204822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)