FastQCFastQC Report
Tue 22 Oct 2024
G187M25.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG187M25.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19895228
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGCTGCACAGAAAGAAGGGGGGAGCAGGGGGCAAAGTTGGGCCATGAA11042395.550270647815648No Hit
AGAGAACTTCTGGTGGATTCACCATGCCTGACCTCAAGCTGTACTACATA7901683.9716458640232726No Hit
GGTGGTGGATGTTTAGCCAGAATGTCAGAAGCTTGGGGCTTTATCTGCGA1426300.7169055815796632No Hit
TGACGACTTCAACAATGACGAAATCACTAAAAAACGTGAAAAATGAGAAA523650.26320381952898453No Hit
CTGAGCAACTGAGAGCTGGTGTTGCTCTTACAGTGGACACAGGTTTGGTT437740.22002261044708812No Hit
GTGCTTCCTGAGACCTGGTCAGAGTTTAAAAGACCACCAATGTTCTCCCA401300.2017066605117569No Hit
CTGGGACGACTTAAAGAGAGGTAAATGCAGGTGAATTCGATCAAAATCTA325380.16354675603617108No Hit
CATGTCCTTGTGGGTCTGGATTACCACACTCAGGATGATATTTTCTGTAC302290.15194095790206577No Hit
ACATGACATGCCCAAAACCATATCTAGAATAGAATCAATCATTGAACCCA295900.14872913243316438No Hit
ATGTCTGGAGCCGAAAACGGTGCTGCCTCAGAAGCTCTGTGGCTCTCGTC237150.11919943817683316No Hit
GCTCACACCAGGACAGAGTCACCAGACTTTATCAGAATGCCAGGGTGTCT215030.10808119414364087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)