FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-CTL-rep-1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-CTL-rep-1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25591968
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT2135350.8343828813790326TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA1100840.430150584745964TruSeq Adapter, Index 7 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA323920.1265709616392143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG669850.057.8325233
TATCGGA42000.057.5981181
CGGAAGA689000.056.685664
ATCGGAA688150.056.4887282
GATCGGA667100.053.016191
GAGCACA871400.044.0828749
AGATCGG382350.043.428741
AGAGCAC907950.042.451738
GAAGAGC924500.041.601226
ACGAGTG523100.041.2165732-33
CGTCTGA671300.041.2041816-17
TCACGAG586900.040.52813730-31
AGTCACG604850.040.3481228-29
CACGTCT684050.040.33078414-15
CTCGTAT624000.039.98531744-45
ATCTCGT636900.039.29264542-43
CACACGT698950.039.05512212-13
GGATATC603300.038.7171538-39
CGTATGC656800.038.5722946-47
TGCCGTC648650.038.19258550-51
Produced by FastQC (version 0.11.2)