FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-CTL-rep-2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-CTL-rep-2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17696803
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC2581411.458687199038154TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG666200.3764521761359947TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT433650.041.19312742-43
TGCCGTC439950.040.3718948-49
CGTATGC446000.040.18818344-45
ATCTCGT446350.039.97302640-41
CCGTCTT446150.039.80656450-51
TATGCCG450950.039.710546-47
ACGGCTA448900.038.71150632-33
TATCGGA15950.036.9864461
TCACGGC469950.036.87230-31
AGTCACG486950.035.83998528-29
TGCTTGA498200.034.81002858-59
CTACATC510950.033.84893436-37
ACATCTC519750.033.40751338-39
GAGCACA897850.032.367399
ATGCCGT430250.032.32283446-47
CGGAAGA901150.032.1593254
TCTCGTA435950.032.07408540-41
GCCGTCT438000.031.73487748-49
CGTCTTC442200.031.43311350-51
TCGTATG448150.031.30102542-43
Produced by FastQC (version 0.11.2)