FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-CTL-rep-3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-CTL-rep-3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15982742
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1259080.7877747135003493TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC234600.028.62909750-51
CCGTCTT238650.028.16314350-51
GCTTGAA235250.027.2178658-59
GCCGTCT247050.027.18634248-49
TGCCGTC256300.026.24222648-49
ATGCCGT257700.026.20146-47
TGCTTGA261200.024.83191558-59
TATGCCG278000.024.5268846-47
CGTATGC282700.024.10231644-45
TCGTATG289500.023.56898542-43
CTCGTAT292200.023.18057642-43
TCTCGTA301100.022.4796340-41
GTATGCC305750.022.36292844-45
ATCTCGT322150.021.0033940-41
CTGCTTG326350.020.82765656-57
GATCTCG329500.020.39077438-39
CGATCTC333700.019.97049938-39
ACGATCT346300.019.37413636-37
GTCTTCT355800.019.24385352-53
CACGATC356050.018.76358436-37
Produced by FastQC (version 0.11.2)