FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-TCP-rep-2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-TCP-rep-2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28609544
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC6033442.1088906555099234TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4273201.493627441248277TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1301650.043.48187642-43
TGCCGTC1304500.043.29200448-49
CGTATGC1314600.043.22675344-45
CCGTCTT1310550.043.06274450-51
TATGCCG1323050.042.9608346-47
ATCTCGT1324700.042.6588440-41
TGCTTGA1382650.040.2424558-59
TGTAATC1406100.039.46407336-37
TAATCTC1423700.039.12531738-39
CTTGTAA1453000.038.28186834-35
ACCTTGT1485750.037.53241332-33
GTCTTCT1512250.037.34684852-53
AGTCACC1532450.036.60950528-29
TATCGGA28000.035.856861
TCACCTT1568350.035.51965330-31
TCTGCTT1621150.034.9198656-57
CCAGTCA1633000.034.79134826-27
CTTCTGC1629900.034.71802554-55
CGTCTGA1674450.034.30389816-17
CTCCAGT1719550.033.1065224-25
Produced by FastQC (version 0.11.2)