FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-TCP-rep-4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-TCP-rep-4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11534057
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353890.3068217887253375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA27150.032.00518844
GATCGGA47450.019.9817844
AGATCGG71250.013.67766544
TAGATCG25250.09.93139443
TCTCGTA10750.09.20839619
CAGATCG30850.09.12741744
GAGATCG38600.08.83305943
CTCGTAT11250.08.799420
TCGTATG12050.07.850122521
AAGATCG25700.07.788846542
CGTATGC13200.07.49952222
TACACGA7201.9375875E-86.7215618
ATCTCGT14800.06.53989718
ACACGAC8103.00206E-86.24629919
CATCTCG15550.06.083029317
CCGTCTT15850.05.968047628
GCCGTCT15850.05.968047627
TGCCGTC17000.05.952716426
ATGCCGT16800.05.89263525
CTACACG9155.862239E-85.769949417
Produced by FastQC (version 0.11.2)