FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-TCP-rep-5.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-TCP-rep-5.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7166996
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA31900.031.23935344
TACACGA8200.021.73029731
ACACGAC8650.020.59867132
TCCGATC9350.019.29286644
CTACACG9100.018.85605230
GATCGGA54450.018.26042443
GCGACCA10500.018.0168743
CACGACG9900.017.55343633
CGACGCT10150.017.3378135
TTCCGAT10800.017.10904743
ACGACGC10300.016.44461634
CGACCAC11900.015.7123894
ACGCTCT11900.015.15783437
GACGCTC11800.015.09987336
ACCGAGA12650.014.7811349
GATCTAC13250.014.60976214
GGCGACC13950.014.0341482
CGCTCTT13650.013.37567538
AGATCGG79500.013.00544544
CCACCGA14800.012.782517
Produced by FastQC (version 0.11.2)