FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-TCP-rep-6.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-TCP-rep-6.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24376823
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1668370.6844083004581852TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT298200.030.7156850-51
CGTCTTC300400.030.40379150-51
GCCGTCT306550.029.8945148-49
ATGCCGT311200.029.50122646-47
TGCCGTC312500.029.37850248-49
CGTATGC334950.027.64331244-45
TCGTATG338350.027.36553242-43
TATGCCG339500.027.28682346-47
CTCGTAT344250.026.79307642-43
GCTTGAA322050.026.76016658-59
TCTCGTA350400.026.25506440-41
TATCTCG367400.024.89158838-39
ATCTCGT374750.024.67580640-41
GTATGCC379050.024.55872744-45
TGCTTGA362050.024.2036658-59
CGATGTA411500.022.00474434-35
CCGATGT430450.021.08565332-33
ACCGATG440400.020.66317432-33
GTATCTC450300.020.34596638-39
CACCGAT451250.020.14529630-31
Produced by FastQC (version 0.11.2)