FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-TCP-rep-7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-TCP-rep-7.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15372454
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT822360.5349568780625397TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT153650.030.4545252-53
GCCGTCT154050.029.86687550-51
TGCCGTC157500.029.72515150-51
CGTCTTC155900.029.4058652-53
ATGCCGT155600.029.38626948-49
CGTATGC166450.028.1553646-47
CTCGTAT168900.027.7750644-45
TATGCCG169950.027.64536548-49
TCGTATG168050.027.60474844-45
TCTCGTA172950.026.6853842-43
ATCTCGT185950.024.96021342-43
GATCTCG187800.024.5879240-41
CGATCTC190250.024.495940-41
GTATGCC192500.024.17258546-47
CCCGATC191800.024.02562538-39
GCTTGAA170350.023.92907160-61
CCGATCT190900.023.91503738-39
TCCCGAT201300.022.72666436-37
GTCCCGA208900.022.2748836-37
CGTCCCG209750.022.26384234-35
Produced by FastQC (version 0.11.2)