FastQCFastQC Report
Sun 6 Aug 2017
CEBPa_All_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPa_All_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161330925
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA44950.011.551831
CGTATGC52350.010.3380544
CTCGTAT53400.09.59935742
TCGTATG58100.09.39065143
TCTCGTA55350.09.06260241
ATCGGAA63400.08.8841322
CGTCTGA70400.08.12470316
ACGTCTG88000.06.424745615
TCGGAAG93250.06.0871433
ATCTCGT89900.05.995752340
ACACGTC103450.05.826679713
CGGAAGA110700.05.2863734
Produced by FastQC (version 0.11.2)