FastQCFastQC Report
Sun 6 Aug 2017
CEBPa_All_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPa_All_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161330925
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG27800.017.48870512
AGCGTCG28800.017.03478610
GCGTCGT29950.016.4540211
GATCGGA43800.011.9128581
GTCGCCG28250.011.760138544
GAGCGTC58800.08.9422499
ATCGGAA62900.08.435732
GTCGTGT63750.08.31655113
TCGTGTA69850.07.716238514
CGTGTAG70750.07.5245415
AGAGCGT75700.07.2370358
TGGTCGC45200.07.155373642
GTGGTCG52700.06.59617141
AAGAGCG87450.06.4660997
TCGGAAG89850.06.27304173
GGTCGCC59350.06.19084143
GATCTCG66400.05.961983734
TCGGTGG84750.05.21732238
CGGAAGA112100.05.1060674
Produced by FastQC (version 0.11.2)