FastQCFastQC Report
Sun 6 Aug 2017
CEBPa_TCPO_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPa_TCPO_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79996656
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG14000.016.65663712
AGCGTCG15400.015.7143510
GCGTCGT16450.014.71120511
GATCGGA22900.010.6718451
GTCGCCG14850.010.37111344
GTCGTGT30600.08.26766613
GAGCGTC30900.08.2589599
TCGTGTA33650.07.975923514
CGTGTAG34950.07.86774115
ATCGGAA32900.07.69607262
TGGTCGC22500.07.5294142
GTGGTCG27600.07.09456541
AGAGCGT44300.06.1585368
AAGAGCG46950.05.9516477
GGTCGCC29750.05.62057143
TCGGAAG50100.05.4933793
GATCTCG32900.05.280935334
Produced by FastQC (version 0.11.2)