FastQCFastQC Report
Sun 6 Aug 2017
CEBPa_Veh_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPa_Veh_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81334269
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA21250.012.4248251
CGTATGC25150.010.93435744
CTCGTAT26400.010.08344742
TCGTATG28950.09.499104543
TCTCGTA27400.09.39443541
ATCGGAA32750.08.9352272
CGTCTGA34300.08.081346516
TCGGAAG45250.06.4666263
ACGTCTG42900.06.46130615
ATCTCGT42950.06.351776640
ACACGTC51050.05.86064813
CGGAAGA53750.05.4437834
Produced by FastQC (version 0.11.2)