FastQCFastQC Report
Sun 6 Aug 2017
CEBPa_Veh_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPa_Veh_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81334269
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG12350.019.4164812
AGCGTCG13850.017.94954710
GCGTCGT14550.016.93464711
GTCGCCG12950.013.08194344
GATCGGA21650.011.7962181
GAGCGTC27100.09.6606059
GTCGTGT30050.09.15108213
ATCGGAA30250.08.6612452
TGGTCGC20850.08.01971342
AGAGCGT35300.07.72873938
CGTGTAG33450.07.694494215
TCGTGTA34450.07.662997714
GTGGTCG26100.07.16509941
AAGAGCG40650.07.14472447
GGTCGCC27600.06.855523643
TCGGAAG44250.06.36876773
GATCTCG32250.06.00121434
CGGAAGA54250.05.1538144
TCGGTGG42050.05.07455838
Produced by FastQC (version 0.11.2)