FastQCFastQC Report
Sun 6 Aug 2017
CEBPb_All_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_All_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161009056
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA45300.010.2483151
ATCGGAA61200.07.9092342
CGTCTGA76200.06.611306716
CGTATGC48900.06.118571844
ACGTCTG91550.05.83918815
TCGGAAG93200.05.759893
CTCGTAT51700.05.489384742
TCGTATG54400.05.459525643
ACACGTC106800.05.067126313
TCTCGTA53200.05.003884341
Produced by FastQC (version 0.11.2)