FastQCFastQC Report
Sun 6 Aug 2017
CEBPb_All_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_All_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161009056
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG26900.015.12961212
AGCGTCG27800.014.95688210
GCGTCGT27900.014.8242811
GATCGGA43600.010.0993971
GTCGCCG30850.09.41403244
GAGCGTC55750.08.0502819
GTCGTGT60350.07.436357513
ATCGGAA61950.07.35688162
CGTGTAG67000.06.63229715
TGGTCGC45250.06.46678242
TCGTGTA68650.06.18476914
GTGGTCG53800.05.929723341
AGAGCGT81200.05.71718368
GGTCGCC55500.05.58962843
GATCTCG65350.05.58656534
TCGGAAG91800.05.20453363
AAGAGCG92750.05.02909477
Produced by FastQC (version 0.11.2)