FastQCFastQC Report
Sun 6 Aug 2017
CEBPb_TCPO_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_TCPO_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78883712
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA23200.011.570421
ATCGGAA29650.09.2759582
CTCGTAT25250.08.19014744
TCTCGTA27200.07.52210443
CGTCTGA39700.07.42539916
ACGTCTG47050.06.21863815
TCGGAAG45150.05.94499643
ACACGTC54900.05.810249313
CGGAAGA54200.05.3985774
ATCTCGT44000.05.25009142
Produced by FastQC (version 0.11.2)