FastQCFastQC Report
Sun 6 Aug 2017
CEBPb_TCPO_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_TCPO_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78883712
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG14500.016.68920712
GCGTCGT14400.016.34713711
AGCGTCG15600.015.37179310
GATCGGA23000.011.1997891
GTCGCCG14800.010.55476344
GAGCGTC28950.09.6511349
GTCGTGT30750.08.51351713
ATCGGAA32700.08.0797972
TCGTGTA33400.07.70630214
CGTGTAG34950.07.553110615
TGGTCGC21500.06.85625242
AGAGCGT39900.06.7273628
GGTCGCC27450.06.011328743
GTGGTCG27200.05.98555941
GATCTCG32550.05.81073734
TCGGAAG47100.05.79652553
AAGAGCG45250.05.78622257
Produced by FastQC (version 0.11.2)