FastQCFastQC Report
Sun 6 Aug 2017
CEBPb_Veh_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_Veh_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82125344
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG13150.015.39104412
GCGTCGT14500.013.95854411
AGCGTCG15200.013.46057610
GATCGGA23250.09.6588241
GTCGCCG15600.08.88529844
GAGCGTC29400.07.932049
GTCGTGT29300.07.43314813
ATCGGAA32300.06.9527922
CGTGTAG34500.06.37631415
TGGTCGC23250.06.1509842
TCGTGTA34650.06.03149614
GTGGTCG25300.06.00035541
AGAGCGT42000.05.4480978
GATCTCG32850.05.42291634
TCGGAAG47200.05.17776783
Produced by FastQC (version 0.11.2)