FastQCFastQC Report
Fri 9 Jun 2017
SRR1177963_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177963_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17897455
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA26600.014.1023535
AATCGTG37500.013.6753555
ATCGTGC43250.012.406856
CGCGTAT4658.15613E-712.2455071
CCACTAG62450.012.1618263
ATCGTAC31050.011.7760156
GGATCGT35900.011.7700214
CTAGCCT82550.011.266461
CGCGGTT23800.011.1650211
CGTGCAT45200.010.9260738
ACTAGCC62100.010.7813635
GGGATCG49800.010.7711083
CTCGATT34150.010.6991311
TCGTGCA51650.010.5729647
AAATCGT47750.010.539364
GAAATCG61250.09.9200783
CGCCGAT17450.09.7893891
CTCGTAT44700.09.7662721
CGATTAG24350.09.7488364
CTCGGGT53650.09.6406261
Produced by FastQC (version 0.11.2)