FastQCFastQC Report
Fri 9 Jun 2017
SRR1177964_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177964_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19342910
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTG44200.015.5823255
CCACTAG75550.013.6430593
ACTAGCC70050.013.4937115
CTAGCCT95250.013.2648751
AAATCGT56700.012.7334784
GATCGTA30000.012.666465
ATCGTGC53100.011.807936
CGTGCAT53050.011.5516688
TCGTGCA61900.011.12797
TAGCCTC105300.010.916252
CGACAAT24000.010.8852391
CGAAAAT45350.010.78813651
GAAATCG73250.010.6994843
CGCAAAT23050.010.509591
CACTAGC95250.010.3226664
CTGAAAT190850.09.9801881
CCCCACT155350.09.9676511
CTCGGTT41100.09.70787051
ATCGTAC39650.09.4640776
CGGAAAT28200.09.4324691
Produced by FastQC (version 0.11.2)