FastQCFastQC Report
Fri 9 Jun 2017
SRR1177965_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177965_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16849678
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA15500.015.3238587
AATCGTG38300.014.0141165
ATCGTGC43200.012.7553166
CTAGCCT78700.012.6752081
CCGCGAT15700.012.4054948
CGATTAG21950.011.9017714
CCACTAG60750.011.8063093
GATCGTA25950.011.7146325
CGCGATG16700.011.662659
AAATCGT45300.011.4290924
CGTGCAT43950.011.349078
CGACAAT30750.011.1223841
GAAATCG59900.010.3086073
ACTAGCC61300.010.3056935
TCGTGCA52200.010.2834257
TAGCCTC90050.010.1808512
CGCGTAT4000.00451789339.500371
ATCGTAC31750.09.2760896
CGTATGC50750.09.26579344-45
CCGCGTT11453.8198777E-119.1269921
Produced by FastQC (version 0.11.2)