FastQCFastQC Report
Fri 9 Jun 2017
SRR1177965_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177965_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16849678
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT83800.013.9282921
AATCGTG36850.013.5316945
CCACTAG65900.013.3997353
ACTAGCC63350.012.6689455
CGACAAT30600.012.55944251
ATCGTGC43050.012.0247756
GATCGTA27700.012.0010335
TAGCCTC92950.011.9481572
AAATCGT44650.011.5908784
GAAATCG56250.011.3940973
CTCGTAT43800.010.9409241
CGTGCAT43150.010.786218
CGATTAG22100.010.7420954
ATCGTAC32000.010.6857786
GGATCGT35950.010.3016554
CACTAGC80650.010.2436844
CGCGTAA4701.496084E-410.095061
TCGTGCA50200.09.9336587
GGGATCG46400.09.9248153
CGCGTAT4902.2770463E-49.6830161
Produced by FastQC (version 0.11.2)