FastQCFastQC Report
Fri 9 Jun 2017
SRR1177979_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177979_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17620163
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT47650.014.0399361
GTCGAAC18050.013.1432171
ACTAGCC56650.013.07564455
CCACTAG65450.012.1128883
CTAGCCT77450.011.2721111
GATCGTA22700.010.877175
CGGCGAT15400.010.7834111
CACTAGC75100.010.4315844
CCGGCAT39850.010.2395061
CTCGTAT43750.09.8689791
TAGCCTC86250.09.8505012
CCCCACT121850.09.6957781
CCGCGAT11850.09.6095221
CGGGTAT27300.09.3851241
CTGAAAT180450.09.2553881
TCGTATG40900.09.1678652
CCGGGAT58000.09.0803771
CGGGAAT33150.09.0170791
CTCCGTT38450.09.0081461
CTGGAAT188100.08.9798711
Produced by FastQC (version 0.11.2)