FastQCFastQC Report
Fri 9 Jun 2017
SRR1177980_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177980_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14431118
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC41400.014.57089244-45
GTCGAAC15500.014.0974471
CGTTATA8700.012.55715754
ATCACGA48800.012.45870534-35
TATGCCG51100.012.176846-47
CGATTAG13800.011.7026054
CTAGCCT56800.011.2901531
CGCGTTT6151.7963066E-710.8135231
CGCAAAT14800.010.59172251
CGTATGA41250.010.4785253
CTGAAAT153700.010.2607371
CCGCAAT13450.010.2421251
CGACAAT26100.010.1920581
TGCCGTC63500.09.91116948-49
CCGGCAT28750.09.7482911
ACTAGCC43600.09.6961275
CTGGAAT157900.09.3861361
GATCGTA17700.09.1243185
CACGATC60600.09.09218236-37
CTCGGAT47050.08.9855511
Produced by FastQC (version 0.11.2)