FastQCFastQC Report
Fri 9 Jun 2017
SRR1177980_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177980_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14431118
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA18800.014.902745
GTCGAAC16400.011.8605581
CGCCGTA23050.011.74960146-47
ACTAGCC43350.011.7210535
CGCGGCA14850.011.181671
CCACTAG51750.010.6421063
CGGGCAT35300.010.48298551
CCGGAAT23250.010.202631
GGATCGT29400.010.0123594
CTCGGAT47450.09.9983621
CTGAAAT150000.09.9628681
CTAGCCT59050.09.9624661
CCGGGAT42900.09.7317391
CGACAAT26600.09.6311291
ATCGTAC30250.09.57582956
CTCCGTT28500.09.4884461
CCGCGAT10002.7102942E-109.4884461
CGGAAAT22500.09.4884451
GGGATCG41600.09.4725043
CGTACTA25800.09.3869058
Produced by FastQC (version 0.11.2)