FastQCFastQC Report
Fri 9 Jun 2017
SRR1177997_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177997_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19746775
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT89950.014.5866961
GATCGTA29050.014.3850485
ACTAGCC65150.014.0675225
CCACTAG75850.012.7091123
TAGCCTC105750.012.214152
CGTATGC60350.011.36969544-45
GGATCGT42200.011.2528444
CGATTAG24600.010.6170124
CGGGAAT28050.010.5077041
GTCGAAC15950.010.431731
CACTAGC97450.010.1844884
CGGGCAT44650.010.0081861
ATCACGA68150.09.89979234-35
GGGATCG53750.09.8066183
CTGAAAT194750.09.7884941
CGCGTTT8802.8085196E-99.7238631
TATGCCG74350.09.64420346-47
CGGAAAC78250.09.59890651
CCCCACT141150.09.5987121
CTCGATT36200.09.4552481
Produced by FastQC (version 0.11.2)