FastQCFastQC Report
Fri 9 Jun 2017
SRR1177997_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177997_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19746775
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC69700.015.7381245
CCACTAG74950.015.3418313
CTAGCCT90400.014.2567241
GATCGTA27800.013.6652935
CGCGTAT4505.3368603E-712.6819951
CGACAAT33750.012.5410851
CTCGTAT61050.011.9185831
TAGCCTC108100.011.7463612
CGACGAT18500.011.5680371
CCCCACT141850.011.5666781
CACTAGC97000.011.5059564
CGACGAC15950.011.0321451
GTCGAAC16400.011.0194181
CGCGTTT7954.802132E-1010.7677341
TGCGTTA14700.010.6587849
TCGTATG55950.010.5399692
GGATCGT40300.010.4884554
CGGAAAT30550.010.2742851
CGGCGAT20050.010.1993611
CGCAGAT36700.010.1075851
Produced by FastQC (version 0.11.2)