FastQCFastQC Report
Fri 9 Jun 2017
SRR1177998_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177998_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23610281
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT111800.016.0374811
ACTAGCC83450.014.2257635
GTCGAAC19100.014.1931271
CGACAAT66200.012.9315651
CCACTAG96600.012.9312313
GATCGTA35750.012.8842045
TAGCCTC136450.012.6703642
CGTATGC83850.011.837934544-45
CACTAGC112650.011.0019894
CCCCACT170900.010.7975751
TATGCCG98550.010.16853846-47
GGATCGT49450.010.0828884
CGGAAAC100500.09.8904671
CGCGGTT21950.09.7502241
CTGAAAT224500.09.49071
CTTAGAT68700.09.4841931
CGAGAAT49050.09.4052281
CTCGGAT76950.09.3944531
GGGATCG64900.09.367563
CCGGCAT46150.09.274861
Produced by FastQC (version 0.11.2)