FastQCFastQC Report
Fri 9 Jun 2017
SRR1177998_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177998_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23610281
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT107700.016.1622751
GTCGAAC18250.015.3753951
ACTAGCC82050.014.9320725
CGACAAT62850.014.7559251
CCACTAG99500.013.2368933
TAGCCTC132650.012.799652
GATCGTA32850.011.9983535
CACTAGC113800.011.4340024
CGCCGAT16850.011.2900851
CTCGTAT66200.010.7763181
CCCCACT171000.010.3462731
CGATTAG27400.010.225664
TCGTATG61050.010.1273272
CTGAAAT223100.09.9339841
CGGAAAC102350.09.8975761
ATCCGCG55200.09.76283440-41
GGATCGT47400.09.4175834
CCGGCAT49500.09.4158161
CGCGTAT5153.6641175E-49.2348511
CGGGAAT34750.09.0328811
Produced by FastQC (version 0.11.2)