FastQCFastQC Report
Fri 9 Jun 2017
SRR1177999_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177999_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21838440
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT99150.015.0124381
ACTAGCC70450.014.1548435
CCACTAG84650.012.3996553
CCGGCAT49700.012.0562921
TAGCCTC119400.011.6548752
GTCGAAC18200.011.4969021
GATCGTA31000.011.4885795
CGGGAAT32700.010.6163381
CGACAAT48450.010.6005791
CACTAGC99850.010.5577564
CGATTAG26850.010.257714
CCCCACT149900.010.0250151
CGCGTTT9205.7661964E-109.82121851
CGGGCAT47000.09.3087121
CGGGGTT39900.09.2965391
CGTATGA66250.09.2480073
CGCAAAT26300.09.2217651
CTCGGAT63200.09.1799941
CACGAAT112800.08.8533941
CTGAAAT217250.08.8215491
Produced by FastQC (version 0.11.2)