FastQCFastQC Report
Fri 9 Jun 2017
SRR1177999_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177999_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21838440
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA27950.014.6118925
ACTAGCC75100.014.2276255
CTAGCCT102100.013.9261271
CCACTAG85650.013.2666973
CGCCGAT15050.012.9548471
GTCGAAC20500.012.2943891
CGCGTAT6251.3897079E-912.1737641
CGGGAAT35700.011.9883451
TAGCCTC115200.011.8471622
CCCCACT150750.011.5453921
CACTAGC99500.011.1206614
CGACAAT47550.011.0008711
CGATTAG24200.010.7930724
GGATCGT42450.010.4040634
CGACGAT18750.010.3984241
CTGAAAT217500.010.3853051
CGAATCG9255.820766E-1110.2797033
CGCGTAG7452.1713277E-810.2128891
CCGGCAT48150.010.0736951
CGGAAAT29300.09.9002721
Produced by FastQC (version 0.11.2)